Carnitine palmitoyltransferase 1 increases lipolysis, UCP1 protein expression and mitochondrial activity in brown adipocytes

Autor: Calderon-Dominguez, María, Sebastián, David, Fucho, Raquel, Weber, Minéia, Mir, Joan F., García-Casarrubios, Ester, Obregón, María Jesús, Zorzano, Antonio, Valverde, Ángela M., Serra, Dolors, Herrero, Laura
Přispěvatelé: Universitat de Barcelona
Předmět:
Physiology
lcsh:Medicine
Biochemistry
Animal Cells
Medicine and Health Sciences
Adipocytes
lcsh:Science
Energy-Producing Organelles
Uncoupling Protein 1
Connective Tissue Cells
Hydrolysis
Carnitina palmitoïl-transferasa 1
Chemical Reactions
Cell Differentiation
Thermogenesis
Lipids
Mitochondria
Chemistry
Adipocytes
Brown
Adipose Tissue
Physiological Parameters
Connective Tissue
Physical Sciences
Brown Adipose Tissue
Obesitat
Cellular Structures and Organelles
Anatomy
Cellular Types
Research Article
Lipolysis
Bioenergetics
Gene Expression Regulation
Enzymologic
Adipocyte Differentiation
Animals
Humans
Obesity
Carnitine O-Palmitoyltransferase
lcsh:R
Body Weight
Biology and Life Sciences
Adipose tissues
Cell Biology
Lipid Metabolism
Rats
Teixit adipós
Biological Tissue
Carnitine palmitoyltransferase I
lcsh:Q
Physiological Processes
Energy Metabolism
Developmental Biology
Zdroj: Recercat. Dipósit de la Recerca de Catalunya
instname
PLoS ONE
PLoS ONE, Vol 11, Iss 7, p e0159399 (2016)
Repositorio Institucional de la Consejería de Sanidad de la Comunidad de Madrid
Consejería de Sanidad de la Comunidad de Madrid
Dipòsit Digital de la UB
Universidad de Barcelona
Popis: The discovery of active brown adipose tissue (BAT) in adult humans and the fact that it is reduced in obese and diabetic patients have put a spotlight on this tissue as a key player in obesity-induced metabolic disorders. BAT regulates energy expenditure through thermogenesis; therefore, harnessing its thermogenic fat-burning power is an attractive therapeutic approach. We aimed to enhance BAT thermogenesis by increasing its fatty acid oxidation (FAO) rate. Thus, we expressed carnitine palmitoyltransferase 1AM (CPT1AM), a permanently active mutant form of CPT1A (the rate-limiting enzyme in FAO), in a rat brown adipocyte (rBA) cell line through adenoviral infection. We found that CPT1AM-expressing rBA have increased FAO, lipolysis, UCP1 protein levels and mitochondrial activity. Additionally, enhanced FAO reduced the palmitate-induced increase in triglyceride content and the expression of obese and inflammatory markers. Thus, CPT1AM-expressing rBA had enhanced fat-burning capacity and improved lipid-induced derangements. This indicates that CPT1AM-mediated increase in brown adipocytes FAO may be a new approach to the treatment of obesity-induced disorders.
Databáze: OpenAIRE
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