A new mechanism for reduced sensitivity to demethylation-inhibitor fungicides in the fungal banana black Sigatoka pathogen Pseudocercospora fijiensis
| Autor: | Diaz‐Trujillo, Caucasella, Chong, Pablo, Stergiopoulos, Ioannis, Cordovez, Viviane, Guzman, Mauricio, De Wit, Pierre J. G. M., Meijer, Harold J. G., Scalliet, Gabriel, Sierotzki, Helge, Lilia Peralta, Esther, Arango Isaza, Rafael E., Kema, Gerrit H. J. |
|---|---|
| Přispěvatelé: | Microbial Ecology (ME) |
| Rok vydání: | 2018 |
| Předmět: |
Crop and Pasture Production
DMI fungicide Pfcyp51 promoter Genotype Plant Biology & Botany Plant Biology Microbiology Promoter Regions Biointeractions and Plant Health Ascomycota Genetic DMI Genetics fungicide Promoter Regions Genetic Fungicides Plant Diseases Musa Original Articles Fungicides Industrial Laboratorium voor Phytopathologie international Laboratory of Phytopathology Original Article Industrial EPS Pfcyp51 promoter |
| Zdroj: | Molecular plant pathology, vol 19, iss 6 Molecular Plant Pathology, 19(6), 1491-1503. John Wiley and Sons Ltd Molecular Plant Pathology 19 (2018) 6 Molecular Plant Pathology, 19(6), 1491-1503 Molecular Plant Pathology |
| ISSN: | 1464-6722 |
| Popis: | The Dothideomycete Pseudocercospora fijiensis, previously Mycosphaerella fijiensis, is the causal agent of black Sigatoka, one of the most destructive diseases of bananas and plantains. Disease management depends on fungicide applications, with a major contribution from sterol demethylation-inhibitors (DMIs). The continued use of DMIs places considerable selection pressure on natural P.fijiensis populations, enabling the selection of novel genotypes with reduced sensitivity. The hitherto explanatory mechanism for this reduced sensitivity was the presence of non-synonymous point mutations in the target gene Pfcyp51, encoding the sterol 14α-demethylase enzyme. Here, we demonstrate a second mechanism involved in DMI sensitivity of P.fijiensis. We identified a 19-bp element in the wild-type (wt) Pfcyp51 promoter that concatenates in strains with reduced DMI sensitivity. A polymerase chain reaction (PCR) assay identified up to six Pfcyp51 promoter repeats in four field populations of P.fijiensis in Costa Rica. We used transformation experiments to swap the wt promoter of a sensitive field isolate with a promoter from a strain with reduced DMI sensitivity that comprised multiple insertions. Comparative invivo phenotyping showed a functional and proportional up-regulation of Pfcyp51, which consequently decreased DMI sensitivity. Our data demonstrate that point mutations in the Pfcyp51 coding domain, as well as promoter inserts, contribute to the reduced DMI sensitivity of P.fijiensis. These results provide new insights into the importance of the appropriate use of DMIs and the need for the discovery of new molecules for black Sigatoka management. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text