Adult T-cell leukaemia/lymphoma-like human T-cell leukaemia virus-1 replication in infective dermatitis

Autor: Gabet, As, Kazanji, M., Couppie, P., Clity, E., Pouliquen, Jf, Sainte-Marie, D., Aznar, C., Wattel, E.
Přispěvatelé: Virologie et pathogenèse virale (VPV), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Laboratoire de Rétrovirologie [Cayenne, Guyane française], Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Service de Dermatologie, Centre Hospitalier Andre Rosemon, Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Coordination Régionale de la lutte contre le Virus de L'Immunodéficience Humaine (COREVIH)-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française]-Université des Antilles (UA), A.S.G. is supported by the Centre Le´on Be´rard. This work was supported by grants from the Ministe`re de l'E´ducation Nationale, de la Recherche et de la Technologie (Programme de Recherche Fondamentale en Microbiologie et Maladies Infectieuses et Parasitaires), from the Association pour la Recherche sur le Cancer, from the Fondation Contre la Leuce´mie, from the Re´gion Rhoˆne-Alpes (programme e´mergence), and from the comite´ de´partemental de la Saoˆne et Loire de la Ligue Nationale Contre le Cancer., Rollin, Bertrand, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2003
Předmět:
Polymerase Chain Reaction/methods
T-Lymphocytes
Blotting
Western
Leukemia-Lymphoma
T-Lymphocytes/*virology
Dermatitis
Acute
Research Support
Antibodies
Viral
Virus Replication
Viral/blood
Antiviral Agents
Polymerase Chain Reaction
Antibodies
Western/methods
immune system diseases
hemic and lymphatic diseases
Parasitic Diseases/immunology/virology
Parasitic Diseases
Humans
Leukemia-Lymphoma
Adult T-Cell
Lamivudine/therapeutic use
Non-U.S. Gov't
OCIS 000.1430
Child
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Antiviral Agents/therapeutic use
Human T-lymphotropic virus 1
HTLV-I-Associated/*immunology/parasitology
Dermatitis/*immunology/parasitology/*virology
Blotting
Viral Load
T-Cell
Clone Cells
Lamivudine
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Regression Analysis
Female
Cell Division
Human T-lymphotropic virus 1/immunology/*physiology
Zdroj: British journal of haematology. 123(3)
ISSN: 0007-1048
Popis: Adult T-cell leukaemia/lymphoma (ATLL) is a malignant T-cell proliferation that occurs in 3-5% of individuals infected with human T-cell leukaemia virus-1 (HTLV-1). HTLV-1 infection is also linked to the development of infective dermatitis (ID), an exudative dermatitis of children that has been proposed as a cofactor of ATLL. Here, HTLV-1 replication was investigated over time in a girl with ID and multiparasitic infestation including strongyloidiasis, a disease also known to predispose HTLV-1 carriers to ATLL. Quantitative polymerase chain reaction (PCR) revealed extremely high proviral loads. During the 2-year period of the present study, the proportion of circulating infected cells ranged between 12% and 36%. Quadruplicate linker-mediated PCR amplification of HTLV-1 flanking sequences identified a pattern of extensive and persistent oligoclonal expansion of infected lymphocytes. As viral loads, both the number and the degree of infected T-cell expansion were independent of treatment or clinical signs. However, the temporal fluctuation of proviral loads correlated significantly with the degree of infected T-cell expansion, but not with the overall number of detected clones. This pattern of HTLV-1 replication over time is very different from that observed in asymptomatic carriers and reminiscent of that observed in ATLL, a result consistent with the proposal of ID as an ATLL cofactor.
Databáze: OpenAIRE
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