New case of c-src gene transduction: the generation of virus PR2257
| Autor: | Dezélée, Philippe, Barnier, Jean-Vianney, Briest'Anská, J, Geryk, Josef, Karakoz, Ivan, Michailik, A, Nehyba, Jiří, Yatsula, Bogdan A., Rynditch, Alla V., Calothy, Georges |
|---|---|
| Přispěvatelé: | CNRS UMR 146 - Institut Curie - Developmental Genetics of Melanocytes (CNRS), Centre National de la Recherche Scientifique (CNRS), Régulations cellulaires et oncogenèse (RCO), Institut Curie [Paris]-Centre National de la Recherche Scientifique (CNRS), PERIGNON, Alain |
| Rok vydání: | 1994 |
| Předmět: |
MESH: Genes
Viral MESH: Humans MESH: Molecular Sequence Data [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior Base Sequence Genes Viral MESH: Avian Sarcoma Viruses [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Molecular Sequence Data [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology [SDV.NEU.PC] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences MESH: Base Sequence MESH: Transduction Genetic MESH: Genes src Genes src Avian Sarcoma Viruses Transduction Genetic Animals Humans MESH: Animals [SDV.NEU.SC] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences |
| Zdroj: | Folia Biologica-Krakow Folia Biologica-Krakow-, Polish Academy of Sciences, 1994, 40 (5), pp.211-23 |
| ISSN: | 0015-5500 0015-5497 |
| Popis: | International audience; PR2257 is a new replication-defective avian sarcoma virus which harbours in addition to the spliced version of the c-src gene also about 950 bp of no-coding cellular sequences located downstream from the c-src stop codon (Geryk et al., 1989). Comparison of the 950 bp region transduced by PR2257 with the chicken c-src cDNA (Dorai et al., 1991) and genomic sequences of the c-src 3' non-coding region from chicken and quail has shown that there are no additional introns. The c-src 3' non-coding region represents the largest c-src exon (No. 12) comprising about 2 kb. Absence of conserved open reading frames within this region in chicken and quail genomic DNAs excludes the possibility for coding a protein by these sequences. Also, the possibility was excluded that numerous endogenous virus-derived sequences identified in molecularly cloned PR2257 provirus played a role in the c-src transduction. After serial passaging of PR2257 virus in vivo a variant PR2257/16 was isolated. In PR2257/16, the size of the env gene was increased due to homologous recombination with a helper virus. In addition to mutations in the viral leader and the v-src coding region, a large deletion in transduced c-src 3' non-coding sequences was found in the PR2257/16 genome. The significance of genome modifications for selective advantage of this viral variant in vivo is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|