Secretome analysis of Trypanosoma cruzi by proteomics studies
| Autor: | Brossas, Jean-Yves, Gulin, Julián Ernesto Nicolás, Bisio, Margarita Maria Catalina, Chapelle, Manuel, Marinach-Patrice, Carine, Bordessoules, Mallaury, Palazon Ruiz, George, Vion, Jeremy, Paris, Luc, Altcheh, Jaime, Mazier, Dominique |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Proteomics
Life Cycles Serum Proteins Cell Lines Protozoan Proteins lcsh:Medicine Ciencias de la Salud Protozoology Biochemistry Heat Shock Response Tandem Mass Spectrometry Chlorocebus aethiops Medicine and Health Sciences Parasitología purl.org/becyt/ford/3.4 [https] lcsh:Science Cellular Stress Responses Protozoans Trypanosoma Cruzi Eukaryota purl.org/becyt/ford/3.1 [https] Bioquímica y Biología Molecular Medicina Básica Cell Processes Protozoan Life Cycles Electrophoresis Polyacrylamide Gel purl.org/becyt/ford/3 [https] Biological Cultures Research Article Neglected Tropical Diseases Trypanosoma CIENCIAS MÉDICAS Y DE LA SALUD Trypanosoma cruzi Tecnologías que involucran la identificación de ADN proteínas y enzimas y cómo influyen en el conjunto de enfermedades y mantenimiento del bienestar Research and Analysis Methods Microbiology Biotecnología de la Salud purl.org/becyt/ford/3.3 [https] Virology parasitic diseases Parasitic Diseases Animals Chagas Disease Vero Cells Protozoan Infections lcsh:R Host Cells Organisms Biology and Life Sciences Proteins Cell Biology Trypomastigotes Tropical Diseases Parasitic Protozoans lcsh:Q Parasitic Disease Viral Transmission and Infection Developmental Biology Chromatography Liquid |
| Zdroj: | PLoS ONE, Vol 12, Iss 10, p e0185504 (2017) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET PLoS ONE |
| DOI: | 10.1371/journal.pone.0185504 |
| Popis: | Background: Chagas disease is a debilitating often fatal disease resulting from infection by the protozoan parasite Trypanosoma cruzi. Chagas disease is endemic in 21 countries of the Americas, and it is an emerging disease in other countries as a result of migration. Given the chronic nature of the infection where intracellular parasites persist for years, the diagnosis of T. cruzi by direct detection is difficult, whereas serologic tests though sensitive may yield false-positive results. The development of new rapid test based on the identification of soluble parasitic antigens in serum would be a real innovation in the diagnosis of Chagas disease. Methods: To identify new soluble biomarkers that may improve diagnostic tests, we investigated the proteins secreted by T. cruzi using mass spectrometric analyses of conditioned culture media devoid of serum collected during the emergence of trypomastigotes from infected Vero cells. In addition, we compared the secretomes of two T. cruzi strains from DTU Tc VI (VD and CL Brener). Results: Analysis of the secretome collected during the emergence of trypomastigotes from Vero cells led to the identification of 591 T. cruzi proteins. Three hundred sixty three proteins are common to both strains and most belong to different multigenic super families (i.e. TcS, GP63, MASP, and DGF1). Ultimately we have established a list of 94 secreted proteins, common to both DTU Tc VI strains that do not belong to members of multigene families. Conclusions: This study provides the first comparative analysis of the secretomes from two distinct T. cruzi strains of DTU TcVI. This led us to identify a subset of common secreted proteins that could potentially serve as serum markers for T. cruzi infection. Their potential could now be evaluated, with specific antibodies using sera collected from patients and residents from endemic regions. Fil: Brossas, Jean Yves. Inserm; Francia. Universite de Paris VI; Francia. Hôpital Pitié-Salpêtrière; Francia Fil: Gulin, Julián Ernesto Nicolás. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas.; Argentina Fil: Bisio, Margarita María Catalina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas.; Argentina Fil: Chapelle, Manuel. Bruker Daltonics; Francia Fil: Marinach Patrice, Carine. Inserm; Francia. Universite de Paris VI; Francia Fil: Bordessoulles, Mallaury. Universite de Paris VI; Francia. Inserm; Francia Fil: Palazon Ruiz, George. Inserm; Francia Fil: Vion, Jeremy. Inserm; Francia Fil: Paris, Luc. Hôpital Pitié-Salpêtrière; Francia. Universite de Paris VI; Francia Fil: Altcheh, Jaime Marcelo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas.; Argentina Fil: Mazier, Dominique. Inserm; Francia. Universite de Paris VI; Francia. Hôpital Pitié-Salpêtrière; Francia |
| Databáze: | OpenAIRE |
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