A Metabolomic Signature of Acute Caloric Restriction
| Autor: | Farooqi, IS, Collet, TH, Sonoyama, T, Henning, E, Keogh, JM, Ingram, B, Kelway, S, Guo, L |
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| Přispěvatelé: | Farooqi, Ismaa [0000-0001-7609-3504], Apollo - University of Cambridge Repository |
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male Obesity and Adipocyte Biology Amino Acids/metabolism Antioxidants/metabolism Caloric Restriction Carbohydrate Metabolism/physiology Chromatography High Pressure Liquid Dehydroepiandrosterone Sulfate/metabolism Endocannabinoids/metabolism Energy Metabolism/physiology Fatty Acids/metabolism Homeostasis Humans Lipid Metabolism/physiology Lipolysis Metabolomics/methods Oxidation-Reduction Steroids/blood Tandem Mass Spectrometry Weight Loss/physiology Dehydroepiandrosterone Sulfate Fatty Acids Lipid Metabolism Antioxidants Weight Loss Carbohydrate Metabolism Metabolomics Steroids Amino Acids Energy Metabolism Clinical Research Articles Endocannabinoids |
| Zdroj: | The Journal of Clinical Endocrinology and Metabolism The Journal of clinical endocrinology and metabolism, vol. 102, no. 12, pp. 4486-4495 |
| ISSN: | 1945-7197 |
| Popis: | Context: The experimental paradigm of acute caloric restriction (CR) followed by refeeding (RF) can be used to study the homeostatic mechanisms that regulate energy homeostasis, which are relevant to understanding the adaptive response to weight loss. Objective: Metabolomics, the measurement of hundreds of small molecule metabolites, their precursors, derivatives, and degradation products, has emerged as a useful tool for the study of physiology and disease and was used here to study the metabolic response to acute CR. Participants, Design, and Setting: We used four ultra high-performance liquid chromatography-tandem mass spectrometry methods to characterize changes in carbohydrates, lipids, amino acids, and steroids in eight normal weight men at baseline, after 48 hours of CR (10% of energy requirements) and after 48 hours of ad libitum RF in a tightly controlled environment. Results: We identified a distinct metabolomic signature associated with acute CR characterized by the expected switch from carbohydrate to fat utilization with increased lipolysis and β-fatty acid oxidation. We found an increase in ω-fatty acid oxidation and levels of endocannabinoids, which are known to promote food intake. These changes were reversed with RF. Several plasmalogen phosphatidylethanolamines (endogenous antioxidants) significantly decreased with CR (all P ≤ 0.0007). Additionally, acute CR was associated with an increase in the branched chain amino acids (all P ≤ 1.4 × 10−7) and dehydroepiandrosterone sulfate (P = 0.0006). Conclusions: We identified a distinct metabolomic signature associated with acute CR. Further studies are needed to characterize the mechanisms that mediate these changes and their potential contribution to the adaptive response to dietary restriction. We conducted a metabolomic analysis of 770 small molecules in eight healthy lean men and identified a distinct metabolomic signature associated with acute caloric restriction. |
| Databáze: | OpenAIRE |
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