Some Synonymous and Nonsynonymous gyrA Mutations in Mycobacterium tuberculosis Lead to Systematic False-Positive Fluoroquinolone Resistance Results with the Hain GenoType MTBDRsl Assays
| Autor: | Ajileye, Adebisi, Alvarez, Nataly, Merker, Matthias, Walker, Timothy M., Akter, Suriya, Brown, Kerstin, Moradigaravand, Danesh, Schön, Thomas, Andres, Sönke, Schleusener, Viola, Omar, Shaheed V., Coll, Francesc, Huang, Hairong, Diel, Roland, Ismail, Nazir, Parkhill, Julian, de Jong, Bouke C., Peto, Tim E. A., Crook, Derrick W., Niemann, Stefan, Robledo, Jaime, Smith, E. Grace, Peacock, Sharon J., Köser, Claudio U. |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Antitubercular Agents
Gene Expression Mycobacterium tuberculosis Microbiology in the medical area Hain GenoType MTBDRsl fluoroquinolones Mechanisms of Resistance DNA Gyrase Drug Resistance Multiple Bacterial Mutation Tuberculosis Multidrug-Resistant Mikrobiologi inom det medicinska området Humans Biological Assay False Positive Reactions Oligonucleotide Probes Phylogeny |
| Zdroj: | Antimicrobial Agents and Chemotherapy |
| ISSN: | 0066-4804 |
| Popis: | In this study, using the Hain GenoType MTBDRsl assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in gyrA in Mycobacterium tuberculosis result in systematic false-resistance results to fluoroquinolones by preventing the binding of wild-type probes. Moreover, such mutations can prevent the binding of mutant probes designed for the identification of specific resistance mutations. Although these mutations are likely rare globally, they occur in approximately 7% of multidrug-resistant tuberculosis strains in some settings. Funding Agencies|Swedish Heart and Lung Foundation; Marianne and Marcus Wallenberg Foundation; Wellcome Trust [201344/Z/16/Z]; NIHR Oxford Biomedical Research Centre; NIHR Oxford Health Protection Research Unit on Healthcare Associated Infection and Antimicrobial Resistance [HPRU-2012-10041]; Health Innovation Challenge Fund [T5-358, HICF-T5-342, WT098600]; German Center for Infection Research (DZIF); European Union TB-PAN-NET [FP7-223681]; PathoNgenTrace [278864]; UK Department of Health; Pacific Biosciences, Inc.; Illumina, Inc.; Colciencias; Hain Lifescience |
| Databáze: | OpenAIRE |
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