Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards

Autor: La Scola, Bernard, Le Bideau, Marion, Andreani, Julien, Hoang, Van Thuan, Grimaldier, Clio, Colson, Philippe, Gautret, Philippe, Raoult, Didier
Přispěvatelé: Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche Biomédicale des Armées (IRBA)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Microbiology (medical)
Pneumonia
Viral
RT-PCR
Specimen Handling
Betacoronavirus
[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Chlorocebus aethiops
Animals
Humans
Viral load
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Pandemics
Vero Cells
[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases
Reverse Transcriptase Polymerase Chain Reaction
SARS-CoV-2
General Medicine
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Patient Discharge
Correlation
Infectious Diseases
SARS-CoV2
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
RNA
Viral
France
Co-culture
Coronavirus Infections
Covid-19
Zdroj: European Journal of Clinical Microbiology and Infectious Diseases
European Journal of Clinical Microbiology and Infectious Diseases, Springer Verlag, 2020, ⟨10.1007/s10096-020-03913-9⟩
European Journal of Clinical Microbiology and Infectious Diseases, 2020, ⟨10.1007/s10096-020-03913-9⟩
European Journal of Clinical Microbiology & Infectious Diseases
ISSN: 0934-9723
1435-4373
DOI: 10.1007/s10096-020-03913-9⟩
Popis: International audience; In a preliminary clinical study, we observed that the combination of hydroxychloroquine and azithromycin was effective against SARS-CoV-2 by shortening the duration of viral load in Covid-19 patients. It is of paramount importance to define when a treated patient can be considered as no longer contagious. Correlation between successful isolation of virus in cell culture and Ct value of quantitative RT-PCR targeting E gene suggests that patients with Ct above 33-34 using our RT-PCR system are not contagious and thus can be discharged from hospital care or strict confinement for non-hospitalized patients.
Databáze: OpenAIRE
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