EMMPRIN (CD147) regulation of MMP-9 in bronchial epithelial cells in COPD

Autor: Jouneau, Stephane, Khorasani, Nadia, de Souza, Patricia, Macedo, Patricia, Zhu, Jie, Bhavsar, Pankaj K, Chung, Kian F
Přispěvatelé: Imperial College London, Signalisation et Réponses aux Agents Infectieux et Chimiques (SeRAIC), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Service de pneumologie [Rennes] = Pneumology [Rennes], CHU Pontchaillou [Rennes], CHU de Rennes, Université de Rennes 1, France- 'Pneumologie développement'- SPLF (Société de Pneumologie de Langue Française)- Imperial College Trust Fund, UK, Université de Rennes (UR)
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Adult
Lipopolysaccharides
Male
MESH: Smoking
matrix metalloproteinase
MESH: Macrophages
Alveolar
Adolescent
Biopsy
Bronchi
MESH: Pulmonary Disease
Chronic Obstructive
extracellular matrix metalloproteinase inducer
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
[SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
chronic obstructive pulmonary disease
Pulmonary Disease
Chronic Obstructive
Young Adult
MESH: Biopsy
MESH: Antigens
CD147
Macrophages
Alveolar
bronchial epithelial cell
MESH: Antibodies
Blocking
MESH: C-Reactive Protein
Humans
Antibodies
Blocking
Cells
Cultured
MESH: Adolescent
MESH: Humans
Smoking
MESH: Bronchi
Epithelial Cells
MESH: Adult
MESH: Matrix Metalloproteinase 9
MESH: Male
respiratory tract diseases
MESH: Leukocytes
Mononuclear
C-Reactive Protein
Matrix Metalloproteinase 9
MESH: Young Adult
MESH: Epithelial Cells
Basigin
Leukocytes
Mononuclear
Female
alveolar macrophage
MESH: Lipopolysaccharides
MESH: Female
MESH: Cells
Cultured
Zdroj: Respirology
Respirology, Wiley, 2011, 16 (4), pp.705-12. ⟨10.1111/j.1440-1843.2011.01960.x⟩
Respirology, 2011, 16 (4), pp.705-12. ⟨10.1111/j.1440-1843.2011.01960.x⟩
ISSN: 1323-7799
1440-1843
DOI: 10.1111/j.1440-1843.2011.01960.x⟩
Popis: International audience; BACKGROUND AND OBJECTIVE: Extracellular matrix metalloproteinase inducer (EMMPRIN or CD147) induces the production of matrix metalloproteinases (MMP) such as MMP-9, which plays an important role in COPD. We determined its cellular origin and role in MMP-9 production in COPD. METHODS: Bronchial biopsies, alveolar macrophages (AM) and blood monocytes (BM) from patients with COPD, healthy smokers and non-smokers, and bronchial epithelial cells (EC) from surgically resected airways from patients with COPD were stimulated with LPS or CRP in the presence and absence of an anti-EMMPRIN blocking antibody. EMMPRIN in BAL, plasma, conditioned media and cell lysates was quantified and immunohistochemical localization of EMMPRIN was determined in bronchial biopsies. MMP-9 activity and mRNA was also determined. RESULTS: EMMPRIN levels in BAL fluid were higher in patients with COPD compared with non-smokers and smokers. There was greater EMMPRIN expression in EC from patients with COPD compared with smokers and non-smokers. EC secreted and expressed more EMMPRIN protein than BM and AM. Blocking EMMPRIN decreased MMP-9 activity in supernatant of EC, but not in those from AM and BM, and decreased MMP-9 mRNA expression in EC. CONCLUSIONS: The increased EMMPRIN expression in COPD is reflected by an increased release from bronchial EC, which are one of the main source of EMMPRIN. EMMPRIN regulates MMP-9 expression in COPD.
Databáze: OpenAIRE
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