Direct degradation of articular cartilage by rheumatoid synovial fluid: contribution of proteolytic enzymes

Autor:	Jp, Larbre, Ar, Moore, Jose Da Silva, Iwamura H, Ioannou Y, Da, Willoughby
Předmět:	Adult Cartilage Articular Male Pancreatic Elastase Middle Aged Arthritis Rheumatoid Iodoacetamide Osteoarthritis Pepstatins Synovial Fluid Humans Female Aged Glycosaminoglycans Peptide Hydrolases
Zdroj:	CIÊNCIAVITAE Europe PubMed Central
Popis:	To test the effects of synovial fluids (SF) on human cartilage in an in vitro model.Freshly collected SF were incubated with cryostat sections of articular cartilage, and glycosaminoglycan (GAG) loss determined by microdensitometry after alcian blue staining.Of 20 rheumatoid SF, 11 induced significant GAG loss compared with only 3 out of 15 osteoarthritic SF. The effect of rheumatoid fluids appeared to be related to disease activity. GAG loss was partially prevented by a broad spectrum serine protease inhibitor and a specific elastase inhibitor. Cartilage degrading activity was lost on storage which may explain why it has not been widely reported before.Rheumatoid SF can directly degrade cartilage through the action of proteases. There is an involvement of serine proteases, elastase in particular.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::8103a3a9de8eb177e3365228d9dc6fc6 http://europepmc.org/abstract/med/7837140 Zobrazit plný text záznamu