Functional characterization of thioredoxin 3 (TRX-3), a Caenorhabditis elegans intestine-specific thioredoxin
| Autor: | Jiménez-Hidalgo, María, Kurz, Cyril Léopold, Pedrajas, José Rafael, Naranjo-Galindo, Francisco José, González-Barrios, María, Cabello, Juan, Sáez, Alberto G., Lozano, Encarnación, Button, Emma L., Veal, Elizabeth A., Fierro-González, Juan Carlos, Swoboda, Peter, Miranda-Vizuete, Antonio |
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| Jazyk: | angličtina |
| Předmět: |
animal structures
Pathogen infection Longevity Original Contribution Stress Biochemistry Photorhabdus luminescens Intestine Thioredoxins Organ Specificity Physiology (medical) Candida albicans Animals Amino Acid Sequence Intestinal Mucosa Caenorhabditis elegans Proteins Caenorhabditis elegans Thioredoxin Oxidation-Reduction |
| Zdroj: | Free Radical Biology & Medicine |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/j.freeradbiomed.2013.11.023 |
| Popis: | Thioredoxins are a class of evolutionarily conserved proteins that have been demonstrated to play a key role in many cellular processes involving redox reactions. We report here the genetic and biochemical characterization of Caenorhabditis elegans TRX-3, the first metazoan thioredoxin with an intestine-specific expression pattern. By using green fluorescent protein reporters we have found that TRX-3 is expressed in both the cytoplasm and the nucleus of intestinal cells, with a prominent localization at the apical membrane. Although intestinal function, reproductive capacity, longevity, and resistance of trx-3 loss-of-function mutants to many stresses are indistinguishable from those of wild-type animals, we have observed a slight reduction in size and a minor reduction in the defecation cycle timing of trx-3 mutants. Interestingly, trx-3 is induced upon infection by Photorhabdus luminescens and Candida albicans, and TRX-3 overexpression provides a modest protection against these pathogens. Together, our data indicate that TRX-3 function in the intestine is dispensable for C. elegans development but may be important to fight specific bacterial and fungal infections. Highlights • trx-3 encodes a novel member of the thioredoxin family in Caenorhabditis elegans. • TRX-3 is the first metazoan thioredoxin specifically expressed in intestinal cells. • TRX-3 is not required for intestine development or intestinal function. • Specific bacterial and fungal infections robustly induce trx-3 expression and TRX-3 overexpression moderately protects against infection. |
| Databáze: | OpenAIRE |
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