A partial metabolic pathway enables group b streptococcus to overcome quinone deficiency in a host bacterial community

Autor: Franza, Thierry, Delavenne, Emilie, Derre-Bobillot, Aurelie, Juillard, Vincent, Boulay, Mylène, Demey, Emmanuelle, Vinh, Joelle, Lamberet, Gilles, Gaudu, Philippe
Přispěvatelé: Université Paris sciences et lettres (PSL), Laboratoire de Spectrométrie de Masse Biologique & Protéomique (SMBP), ESPCI ParisTech-Centre National de la Recherche Scientifique (CNRS), John D. Helmann, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Université de Brest (UBO), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Spectrométrie de Masse Biologique et Protéomique (USR3149 / FRE2032) (SMBP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), PSL Research University (PSL), Ecole Supérieure de Physique et de Chimie Industrielles (ESPCI), Mairie de Paris, Unité de Spectrométrie de Masse Biologique et Protéomique
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Molecular Microbiology
Molecular Microbiology, Wiley, 2016, 102 (1), pp.81-91. ⟨10.1111/mmi.13447⟩
Molecular Microbiology, Wiley, 2016, ⟨10.1111/mmi.13447⟩
ISSN: 0950-382X
1365-2958
DOI: 10.1111/mmi.13447⟩
Popis: International audience; Aerobic respiration metabolism in Group B Streptococcus (GBS) is activated by exogenous heme and menaquinone. This capacity enhances resistance of GBS to acid and oxidative stress and improves its survival. In this work, we discovered that GBS is able to respire in the presence of heme and 1,4-dihydroxy-2-naphthoic acid (DHNA). DHNA is a biosynthetic precursor of demethylmenaquinone (DMK) in many bacterial species. A GBS gene (gbs1789) encodes a homolog of the MenA 1,4-dihydroxy-2-naphthoate prenyltransferase enzyme, involved in the synthesis of demethylmenaquinone. In this study, we showed that gbs1789 is involved in the biosynthesis of long-chain demethylmenaquinones (DMK-10). The Δgbs1789 mutant cannot respire in the presence of heme and DHNA, indicating that endogenously synthesized DMKs are cofactors of the GBS respiratory chain. We also found that isoprenoid side chains from GBS DMKs are produced by the protein encoded by the gbs1783 gene, since this gene can complement an Escherichia coli ispB mutant defective for isoprenoids chain synthesis. In the gut or vaginal microbiote, where interspecies metabolite exchanges occur, this partial DMK biosynthetic pathway can be important for GBS respiration and survival in different niches.
Databáze: OpenAIRE
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