ATP signalling is crucial for the response of human keratinocytes to mechanical stimulation by hypo-osmotic shock

Autor: Nathalie, Azorin, Matthieu, Raoux, Lise, Rodat-Despoix, Thierry, Merrot, Patrick, Delmas, Marcel, Crest
Přispěvatelé: Centre de recherche en neurobiologie - neurophysiologie de Marseille (CRN2M), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Keratinocytes
MESH: Egtazic Acid
Gadolinium
MESH: Thapsigargin
MESH: Chelating Agents
MESH: Calcium Signaling
Mechanotransduction
Cellular
MESH: Receptors
Purinergic P2Y
Ion Channels
Adenosine Triphosphate
Transient Receptor Potential Channels
Osmotic Pressure
Physical Stimulation
MESH: Adenosine Triphosphate
MESH: Adenosine Triphosphatases
Humans
MESH: Animals
Calcium Signaling
MESH: Physical Stimulation
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Egtazic Acid
Cells
Cultured
Chelating Agents
Adenosine Triphosphatases
MESH: Humans
MESH: Mechanotransduction
Cellular
MESH: Transient Receptor Potential Channels
MESH: Osmotic Pressure
MESH: Keratinocytes
MESH: Repressor Proteins
Receptors
Purinergic P2Y
Type C Phospholipases
MESH: Ion Channels
Purinergic P2Y Receptor Antagonists
MESH: Calcium Channels
Thapsigargin
MESH: KCNQ Potassium Channels
MESH: Purinergic P2Y Receptor Antagonists
Calcium Channels
MESH: Pain
MESH: Type C Phospholipases
MESH: Gadolinium
MESH: Cells
Cultured
Zdroj: Experimental Dermatology
Experimental Dermatology, Wiley, 2011, 20 (5), pp.401-7. ⟨10.1111/j.1600-0625.2010.01219.x⟩
Experimental Dermatology, 2011, 20 (5), pp.401-7. ⟨10.1111/j.1600-0625.2010.01219.x⟩
ISSN: 0906-6705
1600-0625
DOI: 10.1111/j.1600-0625.2010.01219.x⟩
Popis: International audience; Touch is detected through receptors located in the skin and the activation of channels in sensory nerve fibres. Epidermal keratinocytes themselves, however, may sense mechanical stimulus and contribute to skin sensation. Here, we showed that the mechanical stimulation of human keratinocytes by hypo-osmotic shock releases adenosine triphosphate (ATP) and increases intracellular calcium. We demonstrated that the release of ATP was found to be calcium independent because emptying the intracellular calcium stores did not cause ATP release; ATP release was still observed in the absence of external calcium and it persisted on chelating cytosolic calcium. On the other hand, the released ATP activated purinergic receptors and mobilized intracellular calcium stores. The resulting depletion of stored calcium led to the activation of capacitative calcium entry. Increase in cytosolic calcium concentration was blocked by the purinergic receptor blocker suramin, phospholipase C inhibitor and apyrase, which hydrolyses ATP. Collectively, our data demonstrate that human keratinocytes are mechanically activated by hypo-osmotic shock, leading first to the release of ATP, which in turn stimulates purinergic receptors, resulting in the mobilization of intracellular calcium and capacitative calcium entry. These results emphasize the crucial role of ATP signalling in the transduction of mechanical stimuli in human keratinocytes.
Databáze: OpenAIRE
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