Physicochemical characterisation of the pertussis vaccine
| Autor: | C, Ceccarini, M, Contorni, P, Costantino, S, D'Ascenzi, E, Gallo, M, Maffei, D, Mannucci, I, Marsili, C, Magagnoli, S, Peppoloni, R, Rappuoli, N, Ravenscroft, S, Ricci |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Pertussis Vaccine
Mice Inbred BALB C tossina Chemical Phenomena Vaccino pertosse immunigenicità risposta immunitaria bambini Chemistry Physical Blotting Western Mice Pertussis Toxin Formaldehyde Chromatography Gel Animals Electrophoresis Polyacrylamide Gel Female Virulence Factors Bordetella Amino Acids Isoelectric Focusing Chromatography High Pressure Liquid |
| Popis: | The characterisation of an acellular pertussis vaccine composed of a genetically modified pertussis toxin, filamentous haemagglutinin and pertactin is described. The three antigens are submitted to a mild treatment with formaldehyde in the presence of lysine before their use in vaccine formulation. Characterisation is performed by amino acid analysis, SDS-PAGE, analytical size exclusion chromatography and, in the case of pertactin, isoelectrofocusing. The effect of some variables on pertactin formaldehyde treatment has been studied by means of isoelectrofocusing and mouse immunogenicity. |
| Databáze: | OpenAIRE |
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