Evaluating resistance to Bt Toxin Cry1Ab by F-2 Screen in European populations of Ostrinia nubilalis (Lepidoptera: Crambidae)
| Autor: | Engels, H, Bourguet, D, Cagan, L, Schuphan, I, Stodola, TJ, Micoud, A, Brazier, C, Mottet, C, Andow, DA, MANACHINI, Barbara Rosy Ines |
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| Přispěvatelé: | Rheinisch-Westfälische Technische Hochschule Aachen University (RWTH), Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Slovak University of Agriculture, Università degli studi di Palermo - University of Palermo, University of Minnesota [Twin Cities] (UMN), University of Minnesota System, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), RWTH Aachen University, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Università degli Studi di Palermo, Engels, H., Bourguet, D., Cagan, L., Manachini, B., Schuphan, I., Stodola, T., Micoud, A., Brazier, C., Mottet, C., Andow, D. |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
Bt maize resistance management [SDV]Life Sciences [q-bio] Drug Resistance Zea mays Hemolysin Proteins Bacterial Proteins Germany Animals European corn borer Bt maize Mon810 resistance management HDR strategy Pest Control Biological Bacillus thuringiensis Toxins Reproduction fungi food and beverages HDR strategy Endotoxins Europe Lepidoptera european corn borer Settore AGR/11 - Entomologia Generale E Applicata Costs and Cost Analysis Female France Mon810 |
| Zdroj: | Journal of Economic Entomology Journal of Economic Entomology, 2010, 103 (5), pp.1803-1809. ⟨10.1603/EC10055⟩ Journal of Economic Entomology, Entomological Society of America, 2010, 103 (5), pp.1803-1809. ⟨10.1603/EC10055⟩ |
| ISSN: | 0022-0493 |
| Popis: | The large-scale cultivation of transgenic crops producing Bacillus thuringiensis (Bt) toxins have already lead to the evolution of Bt resistance in some pest populations targeted by these crops. We used the F2 screening method for further estimating the frequency of resistance alleles of the European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae), to Bt maize, Zea mays L., producing the Cry1Ab toxin. In France, Germany, and Italy, 784, 455, and 80 lines of European corn borer were screened for resistance to Mon810 maize, respectively. In Slovakia, 26 lines were screened for resistance to the Cry1Ab toxin. The cost of F2 screen performed in the four countries varied from U.S. dollars 300 to dollars 1300 per line screened. The major difference in cost was mostly due to a severe loss of univoltine lines during the screen in Germany and Slovakia. In none of the screened lines did we detect alleles conferring resistance to Mon810 maize or to the Cry1Ab toxin. The frequency of resistance alleles were1.0 x 10(-3),1.6 x 10(-3),9.2 x 10(-3), and2.6 x 10(-2) in France, Germany, Italy, and Slovakia, with 95% probability, respectively. The average detection probability over all lines was approximately 90%. Making the assumption that European corn borer populations in these countries belong to the same genetic entity, the frequency of alleles conferring resistance to the Cry1Ab produced by the Mon810 maize in western and central Europe was 1.0 x 10(-4), with a 95% confidence interval of 0-3.0 x 10(-4). |
| Databáze: | OpenAIRE |
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