Structural weakening of the colonic mucus barrier is an early event in ulcerative colitis pathogenesis
| Autor: | van der Post, Sjoerd, Jabbar, Karolina S, Birchenough, George, Arike, Liisa, Akhtar, Noreen, Sjovall, Henrik, Johansson, Malin E V, Hansson, Gunnar C |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Proteomics Time Factors Adolescent Colon Biopsy mucosal protection Young Adult mucus Humans Intestinal Mucosa mucosal barrier ulcerative colitis Aged Retrospective Studies Aged 80 and over Inflammatory Bowel Disease Mucins Colonoscopy respiratory system Middle Aged Colitis Ulcerative Female Follow-Up Studies |
| Zdroj: | Gut |
| ISSN: | 1468-3288 0017-5749 |
| Popis: | Objective The colonic inner mucus layer protects us from pathogens and commensal-induced inflammation, and has been shown to be defective in active UC. The aim of this study was to determine the underlying compositional alterations, their molecular background and potential contribution to UC pathogenesis. Design In this single-centre case–control study, sigmoid colon biopsies were obtained from patients with UC with ongoing inflammation (n=36) or in remission (n=28), and from 47 patients without colonic disease. Mucus samples were collected from biopsies ex vivo, and their protein composition analysed by nanoliquid chromatography-tandem mass spectrometry. Mucus penetrability and goblet cell responses to microbial stimulus were assessed in a subset of patients. Results The core mucus proteome was found to consist of a small set of 29 secreted/transmembrane proteins. In active UC, major structural mucus components including the mucin MUC2 (p |
| Databáze: | OpenAIRE |
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