| Autor: |
O'Flynn K, Russul-Saib M, Ando I, Dl, Wallace, Peter Beverley, Aw, Boylston, Dc, Linch |
| Rok vydání: |
1986 |
| Předmět: |
|
| Zdroj: |
Europe PubMed Central |
| ISSN: |
0019-2805 |
| Popis: |
Antigen-specific T-cell activation is mediated via the CD3-Ti (antigen receptor) complex, and monoclonal antibodies to both CD3 and Ti cause a rapid rise in intracellular Ca2+. This calcium mobilization is not inhibited by monoclonal antibodies to CD2. The rise in calcium mobilization induced by purified PHA (PHA-P) does not occur in a cell line which lacks CD2 expression, and can be blocked in other T cells by anti-CD2 antibodies. A combination of monoclonal antibodies to different epitopes of CD2 causes calcium mobilization and mitogenesis. Reagent grade PHA (PHA-M) induces calcium moblization in cells that lack CD2, and its effects in other T cells cannot be blocked by anti-CD2 antibodies. The effects of PHA-P and PHA-M are thus mediated predominantly through different activation pathways. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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