In-depth analysis of compartmentalization of HIV-1 matrix protein p17 in PBMC and plasma

Autor:	Selleri, M., Dolcetti, R., Caccuri, F., Giombini, E., Rozera, G., Abbate, I., Mammone, A., Zanussi, S., Martorelli, D., SIMONA FIORENTINI, Caruso, A., Capobianchi, M. R.
Rok vydání:	2017
Předmět:	Ultra-deep pyrosequencing Gene Expression Regulation Viral HIV Antigens p17 HIV Infections gag Gene Products Human Immunodeficiency Virus Viral quasispecies HIV-1 matrix protein Compartmentalization HIV-1 Leukocytes Mononuclear Humans Non-Hodgkin lymphoma Phylogeny
Zdroj:	Scopus-Elsevier Europe PubMed Central
ISSN:	1121-7138
Popis:	HIV-1 p17 plays an important role in the virus life-cycle and disease pathogenesis. Recent studies indicated a high heterogeneity of p17. A high number of insertions in the p17 carboxy-terminal region have been more frequently detected in patients with non-Hodgkin lymphoma (NHL), suggesting a role of altered p17 in lymphomagenesis. Based on p17 heterogeneity, possible PBMC/plasma compartmentalization of p17 variants was explored by ultra-deep pyrosequencing in five NHL patients. The high variability of p17 with insertions at the carboxy-terminal region was confirmed in plasma and observed for the first time in proviral genomes. Quasispecies compartmentalization was evident in 4/5 patients. Further studies are needed to define the possible role of p17 quasispecies compartmentalization in lymphomagenesis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::7a364fc6b2378dbaf74752218906249c https://pubmed.ncbi.nlm.nih.gov/28072889 Zobrazit plný text záznamu