Three glycosylated serine-rich repeat proteins play a pivotal role in adhesion and colonization of the pioneer commensal bacterium, Streptococcus salivarius

Autor: Couvigny, Benoit, Lapaque, Nicolas, Rigottier-Gois, Lionel, Guillot, Alain, Chat, Sophie, Meylheuc, Thierry, Kulakauskas, Saulius, Rohde, Manfred, Mistou, Michel-Yves, Renault, Pierre, Dore, Joël, Briandet, Romain, Serror, Pascale, Guédon, Eric
Přispěvatelé: MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris-Saclay, Plateforme MIMA2 Microscopie et Imagerie des Micro-organismes, Animaux et Aliments, Institut National de la Recherche Agronomique (INRA), Helmholtz Centre for Infection Research (HZI), Laboratoire de Sécurité des Aliments, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université Paris-Est (UPE), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Génétique Animale et Biologie Intégrative (GABI), Laboratoire de sécurité des aliments de Maisons-Alfort (LSAl), Ministere de la Recherche et de l'Education Nationale, European Project: 201052,EC:FP7:HEALTH,FP7-HEALTH-2007-A,METAHIT(2008), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Environmental Microbiology
Environmental Microbiology, Wiley-Blackwell, 2017, 19 (9), pp.3579-3594. ⟨10.1111/1462-2920.13853⟩
Environmental Microbiology, 2017, 19 (9), pp.3579-3594. ⟨10.1111/1462-2920.13853⟩
Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2017, 19 (9), pp.3579-3594. ⟨10.1111/1462-2920.13853⟩
Environmental microbiology
ISSN: 1462-2912
1462-2920
DOI: 10.1111/1462-2920.13853⟩
Popis: Sous presse; Bacterial adhesion is a critical step for colonization of the host. The pioneer colonizer and commensal bacterium of the human gastrointestinal tract, Streptococcus salivarius, has strong adhesive properties but the molecular determinants of this adhesion remain uncharacterized. Serine-rich repeat (SRR) glycoproteins are a family of adhesins that fulfill an important role in adhesion. In general, Gram-positive bacterial genomes have a unique SRR glycoprotein-encoding gene. We demonstrate that S. salivarius expresses three large and glycosylated surface-exposed proteins - SrpA, SrpB and SrpC - that show characteristics of SRR glycoproteins and are secreted through the accessory SecA2/Y2 system. Two glycosyltransferases - GtfE/F - encoded outside of the secA2/Y2 locus, unusually, perform the first step of the sequential glycosylation process, which is crucial for SRR activity. We show that SrpB and SrpC play complementary adhesive roles involved in several steps of the colonization process: auto-aggregation, biofilm formation and adhesion to a variety of host epithelial cells and components. We also show that at least one of the S. salivarius SRR glycoproteins is important for colonization in mice. SrpA, SrpB and SrpC are the main factors underlying the multifaceted adhesion of S. salivarius and therefore play a major role in host colonization.
Databáze: OpenAIRE
Externí odkaz: