Ethanol inhibits the binding of substance P to rat brain cortex NK1 receptors

Autor: Scarrone, S, Cappelli, A, Cupello, A, Matarrese, M, Moresco, RM, Fazio, F.
Přispěvatelé: Scarrone, S, Cappelli, A, Cupello, A, Matarrese, M, Moresco, R, Fazio, F
Rok vydání: 2003
Předmět:
Zdroj: Neurochemical research 28 (2003): 1159–1162.
info:cnr-pdr/source/autori:Scarrone S., Cappelli A., Cupello A., Matarrese M., Moresco R.M., Fazio F./titolo:Ethanol inhibits the binding of substance P to rat brain cortex NK1 receptors./doi:/rivista:Neurochemical research/anno:2003/pagina_da:1159/pagina_a:1162/intervallo_pagine:1159–1162/volume:28
ISSN: 0364-3190
Popis: The binding of 125I-labeled substance P (SP) to rat brain cortex membranes has been studied under control conditions and in the presence of ethanol. The binding of SP at low concentrations (20-1000 pM) gave two components, one with a KD value of 80 pM and another one with a KD of 500 pM. The higher-affinity component is due to NK1 receptors, as confirmed by the inhibition of the SP binding by the rodent NK1 specific agonist [Sar9 Met(O2)11]SP. Ethanol (1.7 mM) added to the binding assays inhibited by more than 50% the specific binding at a very low SP concentration (20 pM); however, it had no effect at SP concentrations ranging from 50 to 120 pM. This suggests a decrease by ethanol of the affinity of SP to the NK1 receptors involved in this binding component. The ethanol effect disappeared at [EtOH] < or = 0.17 mM.
Databáze: OpenAIRE
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