Molecular gut content analysis of different spider body parts
| Autor: | Macías Hernández, Nuria, Athey, K., Tonzo, Vanina, Wangensteen Fuentes, Owen S. (Simon), Arnedo Lombarte, Miquel Àngel, Harwood, J. D. |
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| Přispěvatelé: | Universitat de Barcelona, Finnish Museum of Natural History |
| Předmět: |
Male
Time Factors GENUS DYSDERA Predation lcsh:Medicine Artificial Gene Amplification and Extension INTRAGUILD PREDATION Zoological taxonomy Polymerase Chain Reaction Database and Informatics Methods Sequencing techniques DNA sequencing Aranyes Molècules lcsh:Science DNA extraction Data Management DETECTABILITY HALF-LIFE CANARY-ISLANDS VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473 Ecology PHYLOGENETIC UTILITY Eukaryota Spiders Gastrointestinal Contents Trophic Interactions Community Ecology 1181 Ecology evolutionary biology Digestion Female Sequence Analysis Research Article Computer and Information Sciences Food Chain Arthropoda Bioinformatics Sequence Databases Taxonomia zoològica Research and Analysis Methods complex mixtures Extraction techniques PREY DNA Arachnida DNA DETECTION SUCCESS VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 Animals DNA Barcoding Taxonomic Molecular Biology Techniques Molecular Biology Taxonomy VDP::Mathematics and natural science: 400::Basic biosciences: 470::Molecular biology: 473 WOLF SPIDER APHID PREDATION Ecology and Environmental Sciences lcsh:R Organisms Biology and Life Sciences DNA Feeding Behavior Molecules Invertebrates VDP::Mathematics and natural science: 400::Basic biosciences: 470 Biological Databases Predatory Behavior GENERALIST PREDATORS lcsh:Q Sequence Alignment |
| Zdroj: | Recercat. Dipósit de la Recerca de Catalunya instname PLoS ONE, Vol 13, Iss 5, p e0196589 (2018) PLoS ONE Dipòsit Digital de la UB Universidad de Barcelona |
| Popis: | Source at: http://doi.org/10.1371/journal.pone.0196589 Molecular gut-content analysis has revolutionized the study of food webs and feeding interactions, allowing the detection of prey DNA within the gut of many organisms. However, successful prey detection is a challenging procedure in which many factors affect every step, starting from the DNA extraction process. Spiders are liquid feeders with branched gut diverticula extending into their legs and throughout the prosoma, thus digestion takes places in different parts of the body and simple gut dissection is not possible. In this study, we investigated differences in prey detectability in DNA extracts from different parts of the spider´s body: legs, prosoma and opisthosoma, using prey-specific PCR and metabarcoding approaches. We performed feeding trials with the woodlouse hunter spider Dysdera verneaui Simon, 1883 (Dysderidae) to estimate the time at which prey DNA is detectable within the predator after feeding. Although we found that all parts of the spider body are suitable for gut-content analysis when using prey-specific PCR approach, results based on metabarcoding suggested the opisthosoma is optimal for detection of predation in spiders because it contained the highest concentration of prey DNA for longer post feeding periods. Other spiders may show different results compared to D. verneaui, but given similarities in the physiology and digestion in different families, it is reasonable to assume this to be common across species and this approach having broad utility across spiders. |
| Databáze: | OpenAIRE |
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