The ABCs of viral hepatitis that define biomarker signatures of acute viral hepatitis

Autor: Duffy, Darragh, Mamdouh, Rasha, Laird, Melissa, Soneson, Charlotte, Le Fouler, Lenaig, El-Daly, Maï, Casrouge, Armanda, Decalf, Jérémie, Abbas, Amal, Eldin, Noha Sharaf, Fontes, Magnus, Abdel-Hamid, Mohamed, Mohamed, Mostafa K., Rafik, Mona, Fontanet, Arnaud, Albert, Matthew L.
Přispěvatelé: Immunobiologie des Cellules Dendritiques, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Faculty of medicine, Université Ain Shams, Centre for Mathematical Sciences, Mathematical Statistics, Lund University [Lund], Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), liver diseases research unit, National Hepatology & Tropical Medicine Research Institute, Menoufia University, National Liver Institute [Menoufia, Egypt], Menoufia University [Egypte]-Menoufia University [Egypte], Minia University, Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), Université Paris Descartes - Paris 5 (UPD5), This work was supported by ANRS grant 12199 (to M.L.A., A.F., and M.R.), the European Research Council Young Investigator Award (to M.L.A.), and the European FP7 project SPHINX (grant reference no.: 261365), European Project: 261365,EC:FP7:HEALTH,FP7-HEALTH-2010-single-stage,SPHINX(2010), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM), Epidémiologie des Maladies Emergentes, Pasteur-Cnam risques infectieux et émergents (PACRI), National Liver Institute
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Hepatology
Hepatology, 2014, 59 (4), pp.1273-1282. ⟨10.1002/hep.26901⟩
Hepatology, Wiley-Blackwell, 2014, 59 (4), pp.1273-1282. ⟨10.1002/hep.26901⟩
Hepatology; Vol 59
ISSN: 0270-9139
1527-3350
DOI: 10.1002/hep.26901⟩
Popis: Viral hepatitis is the leading cause of liver disease worldwide and can be caused by several agents, including hepatitis A (HAV), B (HBV), and C (HCV) virus. We employed multiplexed protein immune assays to identify biomarker signatures of viral hepatitis in order to define unique and common responses for three different acute viral infections of the liver. We performed multianalyte profiling, measuring the concentrations of 182 serum proteins obtained from acute HAV- (18), HBV- (18), and HCV-infected (28) individuals, recruited as part of a hospital-based surveillance program in Cairo, Egypt. Virus-specific biomarker signatures were identified and validation was performed using a unique patient population. A core signature of 46 plasma proteins was commonly modulated in all three infections, as compared to healthy controls. Principle component analysis (PCA) revealed a host response based upon 34 proteins, which could distinguish HCV patients from HAV- and HBV-infected individuals or healthy controls. When HAV and HBV groups were compared directly, 34 differentially expressed serum proteins allowed the separation of these two patient groups. A validation study was performed on an additional 111 patients, confirming the relevance of our initial findings, and defining the 17 analytes that reproducibly segregated the patient populations.This combined discovery and biomarker validation approach revealed a previously unrecognized virus-specific induction of host proteins. The identification of hepatitis virus specific signatures provides a foundation for functional studies and the identification of potential correlates of viral clearance.
Databáze: OpenAIRE
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