Assessing anesthetic activity through modulation of the membrane dipole potential
Autor: | Davis, BM, Brenton, J, Davis, S, Shamsher, E, Sisa, C, Grgic, L, Cordeiro, MF |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
Biochemistry & Molecular Biology
retina RETINAL GANGLION-CELLS MEYER-OVERTON HYPOTHESIS Membrane Fluidity model membranes QD415-436 di-8-ANEPPs Biochemistry diagnostic tools Cell Line Membrane Potentials GENERAL-ANESTHESIA 1101 Medical Biochemistry And Metabolomics INHALED ANESTHETICS IN-VIVO Research Articles Anesthetics Neurons lipid rafts Science & Technology Dose-Response Relationship Drug RECEPTOR FUNCTION AUDIT PROJECT NAP5 0601 Biochemistry And Cell Biology cholesterol fluorescence spectroscopy eye PHOSPHOLIPID-MEMBRANES accidental awareness during general anesthesia LONG-CHAIN ALCOHOLS Liposomes Life Sciences & Biomedicine ACCIDENTAL AWARENESS |
Zdroj: | Journal of Lipid Research, Vol 58, Iss 10, Pp 1962-1976 (2017) Journal of Lipid Research |
ISSN: | 0022-2275 |
Popis: | There is great individual variation in response to general anesthetics (GAs) leading to difficulties in optimal dosing and sometimes even accidental awareness during general anesthesia (AAGA). AAGA is a rare, but potentially devastating, complication affecting between 0.1% and 2% of patients undergoing surgery. The development of novel personalized screening techniques to accurately predict a patient’s response to GAs and the risk of AAGA remains an unmet clinical need. In the present study, we demonstrate the principle of using a fluorescent reporter of the membrane dipole potential, di-8-ANEPPs, as a novel method to monitor anesthetic activity using a well-described inducer/noninducer pair. The membrane dipole potential has previously been suggested to contribute a novel mechanism of anesthetic action. We show that the fluorescence ratio of di-8-ANEPPs changed in response to physiological concentrations of the anesthetic, 1-chloro-1,2,2-trifluorocyclobutane (F3), but not the structurally similar noninducer, 1,2-dichlorohexafluorocyclobutane (F6), to artificial membranes and in vitro retinal cell systems. Modulation of the membrane dipole provides an explanation to overcome the limitations associated with the alternative membrane-mediated mechanisms of GA action. Furthermore, by combining this technique with noninvasive retinal imaging technologies, we propose that this technique could provide a novel and noninvasive technique to monitor GA susceptibility and identify patients at risk of AAGA. |
Databáze: | OpenAIRE |
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