A Luciferase Reporter for Gene Expression Studies and Dynamic Imaging of Superficial Candida albicans Infections
| Autor: | Pietrella, Donatella, Enjalbert, Brice, Zeidler, Ute, Znaidi, Sadri, Rachini, Anna, Vecchiarelli, Anna, d'Enfert, Christophe |
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| Přispěvatelé: | Experimental Medicine and Biochemical Sciences, Università degli Studi di Perugia (UNIPG), University of Aberdeen, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Biologie et Pathogénicité fongiques, Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris], Università degli Studi di Perugia = University of Perugia (UNIPG), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Biologie et Pathogénicité fongiques (BPF), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Gene Expression Profiling
Superficial infection Vulvovaginal candidiasis Candidiasis Cutaneous Imaging Mice Genes Reporter Gaussia princeps [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Candida albicans Host-Pathogen Interactions Luminescent Measurements Vagina Subcutaneous candidiasis Animals Female Luciferases Luciferase Reporter Gene fusion [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology |
| Zdroj: | Methods in Molecular Biology Methods in Molecular Biology, Humana Press/Springer Imprint, 2012, Host-Fungus Interactions. Methods and Protocols, 845, pp.537-546. ⟨10.1007/978-1-61779-539-8_39⟩ Methods in Molecular Biology, 2012, Host-Fungus Interactions. Methods and Protocols, 845, pp.537-546. ⟨10.1007/978-1-61779-539-8_39⟩ |
| ISSN: | 1064-3745 1940-6029 |
| Popis: | International audience; Real-time imaging of fungal infections is becoming integral to the study of host-pathogen interactions, as it allows monitoring of the spatial and temporal progression of pathogen growth or of the host response in a single animal as well as reducing the number of animals used to obtain signifi cant data. We present different applications of a novel luciferase reporter gene constructed from the coding sequences of the Candida albicans PGA59 gene, encoding a GPI-linked cell wall protein, and the Gaussia princeps luciferase gene. Upon addition of the coelenterazine substrate, light produced by the surface-exposed luciferase can be used to quantify gene expression from a variety of C. albicans promoters as well as monitoring cutaneous, subcutaneous, and vaginal infections. |
| Databáze: | OpenAIRE |
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