Next generation histology methods for three-dimensional imaging of fresh and archival human brain tissues
| Autor: | Lai, Hei Ming, Liu, Alan King Lun, Ng, Harry Ho Man, Goldfinger, Marc H., Chau, Tsz Wing, DeFelice, John, Tilley, Bension S., Wong, Wai Man, Wu, Wutian, Gentleman, Steve M. |
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| Přispěvatelé: | Parkinson's UK, The Jean Shanks Foundation |
| Rok vydání: | 2018 |
| Předmět: |
Science & Technology
Paraffin Embedding BASAL FOREBRAIN Science Brain Fluorescent Antibody Technique Immunohistochemistry Article Multidisciplinary Sciences ALZHEIMERS-DISEASE Catecholamines Imaging Three-Dimensional SYSTEMS Science & Technology - Other Topics Humans lcsh:Q RECONSTRUCTION CONNECTIONS lcsh:Science Author Correction NEURONS |
| Zdroj: | Nature Communications Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018) NATURE COMMUNICATIONS |
| Popis: | Modern clearing techniques for the three-dimensional (3D) visualisation of neural tissue microstructure have been very effective when used on rodent brain but very few studies have utilised them on human brain material, mainly due to the inherent difficulties in processing post-mortem tissue. Here we develop a tissue clearing solution, OPTIClear, optimised for fresh and archival human brain tissue, including formalin-fixed paraffin-embedded material. In light of practical challenges with immunostaining in tissue clearing, we adapt the use of cresyl violet for visualisation of neurons in cleared tissue, with the potential for 3D quantification in regions of interest. Furthermore, we use lipophilic tracers for tracing of neuronal processes in post-mortem tissue, enabling the study of the morphology of human dendritic spines in 3D. The development of these different strategies for human tissue clearing has wide applicability and, we hope, will provide a baseline for further technique development. Current available tissue clearing techniques are mostly used for rodent tissues. Here, the authors develop OPTIClear solution for fresh and archival human brain tissue clearing and establish associated protocols for three-dimensional histological investigations. |
| Databáze: | OpenAIRE |
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