A Potential Antineoplastic Peptide of Human Prostate Cancer Cells Derived from the Lesser Spotted Dogfish (Scyliorhinus canicula L.)
| Autor: | Bosseboeuf, Adrien, Baron, Amandine, Duval, Elise, Gautier, Aude, Sourdaine, Pascal, Auvray, Pierrick |
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| Přispěvatelé: | Université de Caen Normandie (UNICAEN), Normandie Université (NU), C.RIS Pharma, Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
lesser spotted dogfish (scyliorhinus canicula) lesser spotted dogfish (Scyliorhinus canicula) Cell Death antineoplastic [SDV]Life Sciences [q-bio] Prostatic Neoplasms Antineoplastic Agents Middle Aged marine peptide Article Pyrrolidonecarboxylic Acid lcsh:Biology (General) Dogfish Cell Line Tumor Animals Humans cancer Peptides lcsh:QH301-705.5 Cytoskeleton mda-pca-2b ComputingMilieux_MISCELLANEOUS Cell Proliferation |
| Zdroj: | Marine drugs Marine drugs, MDPI, 2019, 17 (10), pp.585. ⟨10.3390/md17100585⟩ Marine Drugs, Vol 17, Iss 10, p 585 (2019) Marine Drugs Volume 17 Issue 10 |
| ISSN: | 1660-3397 |
| DOI: | 10.3390/md17100585⟩ |
| Popis: | The purpose of the present paper is to investigate the mechanism of action of a pyroglutamate-modified peptide (pE-K092D) on in vitro growth inhibition of MDA-Pca-2b prostate cancer cells. This peptide was derived from a peptide previously isolated from the testis of the lesser spotted dogfish and identified as QLTPEALADEEEMNALAAR (K092D). The effect of the peptide on cell proliferation and cell death mechanisms was studied by flow cytometry. Cellular morphology and cytoskeleton integrity of peptide-treated cells were observed by immunofluorescence microscopy. Results showed the onset of peptide induced early cytoskeleton perturbation, inhibition of autophagy, inhibition of cell proliferation and, at the end, non-apoptotic cell death mechanisms (membrane destabilization and necrosis). All those mechanisms seem to contribute to MDA-Pca-2b growth inhibition by a main cytostatic fate. |
| Databáze: | OpenAIRE |
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