Intravascular Application of Labelled Cell Spheroids: An Approach for Ischemic Peripheral Artery Disease
| Autor: | Schmehl, Jörg, Stoll, Hartmut, Danalache, Marina, Grözinger, Gerd Christian, Greiner, Tim-Oliver, Leibfritz, Rebecca Felizitas, Martirosian, Petros, Nikolaou, Konstantin, Elser, Stefanie |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
critical limb ischemia
mesenchymal stem cells Staining and Labeling QH301-705.5 Cell Culture Techniques endovascular application regenerative medicine Cell Differentiation Mesenchymal Stem Cell Transplantation peripheral artery disease Article Peripheral Arterial Disease Chemistry Ischemia Spheroids Cellular Animals Humans magnetic resonance imaging small particles of iron oxide 3D spheroids Biology (General) QD1-999 |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 6831, p 6831 (2021) International Journal of Molecular Sciences Volume 22 Issue 13 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Mesenchymal stem cells (MSC) are known for their vascular regeneration capacity by neoangiogenesis. Even though, several delivery approaches exist, particularly in the case of intravascular delivery, only limited number of cells reach the targeted tissue and are not able to remain on site. Applicated cells exhibit poor survival accompanied with a loss of functionality. Moreover, cell application techniques lead to cell death and impede the overall MSC function and survival. 3D cell spheroids mimic the physiological microenvironment, thus, overcoming these limitations. Therefore, in this study we aimed to evaluate and assess the feasibility of 3D MSCs spheroids for endovascular application, for treatment of ischemic peripheral vascular pathologies. Multicellular 3D MSC spheroids were generated at different cell seeding densities, labelled with ultra-small particles of iron oxide (USPIO) and investigated in vitro in terms of morphology, size distribution, mechanical stability as well as ex vivo with magnetic resonance imaging (MRI) to assess their trackability and distribution. Generated 3D spheroids were stable, viable, maintained stem cell phenotype and were easily trackable and visualized via MRI. MSC 3D spheroids are suitable candidates for endovascular delivery approaches in the context of ischemic peripheral vascular pathologies. |
| Databáze: | OpenAIRE |
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