| Popis: |
Dry MeOH extracts of the twig barks ofPyrus communissubsp.pyraster,P. spinosaand their hybridP.×jordanoviinothosubsp.velenovskyi, collected in wild in Serbia, were analyzed. By LC/MS, the contents of arbutin (99.9–131.0 mg/g), chlorogenic acid (2.2–6.3 mg/g), catechin (1.0–5.3 mg/g) and total dimeric and trimeric procyanidins (42.2–61.3 mg/g), including procyanidin B2 (8.9–17.2 mg/g), were determined. Colorimetrically, high contents of total phenolics (436.2–533.4 mg GAE/g) and tannins (339.4–425.7 mg GAE/g), as well as strong total antioxidant activities (FRAP values 4.5–5.9 mmol Fe2+/g), and DPPH (SC50=6.6–7.1 μg/ml) and hydroxyl radical (SC50=447.1–727.7 μg/ml) scavenging abilities were revealed.In vitro, all extracts exhibited notable inhibition ofα-amylase (IC50=310.8–617.7 μg/ml) and particularly strong inhibition ofα-glucosidase (IC50=2.1–3.7 μg/ml). Molecular docking predicted that among identified compounds procyanidin B2 is the best inhibitor of these carbohydrate-digesting enzymes. Obtained results showed that the barks of investigatedPyrushybrid and its parent taxa have similar composition and bioactivity. |
