Dna methylation levels in mononuclear leukocytes from the mother and her child are associated with ige sensitization to allergens in early life
| Autor: | Acevedo, N., Scala, G., Merid, S.K., Frumento, P., Bruhn, S., Andersson, A., Ogris, C., Bottai, M., Pershagen, G., Koppelman, G.H., Melén, E., Sonnhammer, E., Alm, J., Söderhäll, C., Kere, J., Greco, D., Scheynius, A. |
|---|---|
| Přispěvatelé: | STEMM - Stem Cells and Metabolism Research Program, Research Programs Unit, University of Helsinki, Institute of Biotechnology, Acevedo, Nathalie, Scala, Giovanni, Merid, Simon Kebede, Frumento, Paolo, Bruhn, Sören, Andersson, Anna, Ogris, Christoph, Bottai, Matteo, Pershagen, Göran, Koppelman, Gerard H, Melén, Erik, Sonnhammer, Erik, Alm, Johan, Söderhäll, Cilla, Kere, Juha, Greco, Dario, Scheynius, Annika, Groningen Research Institute for Asthma and COPD (GRIAC), Tampere University, BioMediTech |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
maternal effect
Adult Male Maternal effects Cells IgE sensitization Mononuclear Mothers ALLADIN Allergens Atopy BAMSE DNA methylation Epigenetics Cells Cultured Child Preschool Cohort Studies CpG Islands Female Fetal Blood Genetic Predisposition to Disease Humans Hypersensitivity Immunoglobulin E Leukocytes Mononuclear Pregnancy DNA Methylation Article lcsh:Chemistry Leukocytes Alladin Bamse Dna Methylation Ige Sensitization Maternal Effects Child Preschool lcsh:QH301-705.5 Mother Cultured Allergen lcsh:Biology (General) lcsh:QD1-999 1182 Biochemistry cell and molecular biology 3111 Biomedicine Cohort Studie CpG Island epigenetic Human |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 801, p 801 (2021) International Journal of Molecular Sciences, 22(2):801. MDPI AG Int. J. Mol. Sci. 22:801 (2021) |
| ISSN: | 1422-0067 |
| Popis: | DNA methylation changes may predispose becoming IgE-sensitized to allergens. We analyzed whether DNA methylation in peripheral blood mononuclear cells (PBMC) is associated with IgE sensitization at 5 years of age (5Y). DNA methylation was measured in 288 PBMC samples from 74 mother/child pairs from the birth cohort ALADDIN (Assessment of Lifestyle and Allergic Disease During INfancy) using the HumanMethylation450BeadChip (Illumina). PBMCs were obtained from the mothers during pregnancy and from their children in cord blood, at 2 years and 5Y. DNA methylation levels at each time point were compared between children with and without IgE sensitization to allergens at 5Y. For replication, CpG sites associated with IgE sensitization in ALADDIN were evaluated in whole blood DNA of 256 children, 4 years old, from the BAMSE (Swedish abbreviation for Children, Allergy, Milieu, Stockholm, Epidemiology) cohort. We found 34 differentially methylated regions (DMRs) associated with IgE sensitization to airborne allergens and 38 DMRs associated with sensitization to food allergens in children at 5Y (Sidak p ≤ 0.05). Genes associated with airborne sensitization were enriched in the pathway of endocytosis, while genes associated with food sensitization were enriched in focal adhesion, the bacterial invasion of epithelial cells, and leukocyte migration. Furthermore, 25 DMRs in maternal PBMCs were associated with IgE sensitization to airborne allergens in their children at 5Y, which were functionally annotated to the mTOR (mammalian Target of Rapamycin) signaling pathway. This study supports that DNA methylation is associated with IgE sensitization early in life and revealed new candidate genes for atopy. Moreover, our study provides evidence that maternal DNA methylation levels are associated with IgE sensitization in the child supporting early in utero effects on atopy predisposition. publishedVersion |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|