The α-hydroxyketone LAI-1 regulates motility, Lqs-dependent phosphorylation signalling and gene expression of Legionella pneumophila

Autor: Schell, Ursula, Simon, Sylvia, Sahr, Tobias, Hager, Dominik, Albers, Michael F., Kessler, Aline, Fahrnbauer, Felix, Trauner, Dirk, Hedberg, Christian, Buchrieser, Carmen, Hilbi, Hubert
Přispěvatelé: Max Von Pettenkofer Institute (MVP), Ludwig-Maximilians-Universität München (LMU), Institute of Medical Microbiology [Zurich], Universität Zürich [Zürich] = University of Zurich (UZH), Biologie des Bactéries intracellulaires - Biology of Intracellular Bacteria, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Department of Chemistry, LMU University of Munich, University of Munich, Chemical Biology Center (KBC), Umeå University, Work in the group of H.H. was supported by the German Research Foundation (DFG, HI 1511/2-1, HI 1511/5-1) and the Swiss National Science Foundation (SNF, 31003A_153200). Work in the C.B. laboratory was financed by the Institut Pasteur, the Institut Carnot-Pasteur MI, the French Region Ile de France (DIM Malinf),the grant N°ANR-10-LABX-62-IBEID, the Fondation pour la Recherche Médicale (FRM) grant N° DEQ20120323697 and the Pasteur-Weizmann consortium ‘The roles of non-codingRNAs in regulation of microbial life styles and virulence’. C.H. and M.F.A thank the Knut and Alice Wallenberg foundation (Sweden) for generous support, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), University of Zurich, Hilbi, Hubert, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
MESH: Signal Transduction
MESH: Gene Expression
Movement
viruses
Gene Expression
610 Medicine & health
MESH: Ketones
MESH: Movement
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
MESH: Quorum Sensing
Legionella pneumophila
MESH: Gene Expression Profiling
Cell Movement
Alkanes
1312 Molecular Biology
Escherichia coli
Phosphorylation
Vibrio cholerae
MESH: Cell Movement
MESH: Gene Expression Regulation
Bacterial
MESH: Phosphorylation
10179 Institute of Medical Microbiology
MESH: Escherichia coli
Gene Expression Profiling
2404 Microbiology
MESH: Host-Pathogen Interactions
Quorum Sensing
Gene Expression Regulation
Bacterial
MESH: Transcription Factors
Ketones
MESH: Legionella pneumophila
MESH: Alkanes
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Host-Pathogen Interactions
570 Life sciences
biology
MESH: Vibrio cholerae
Signal Transduction
Transcription Factors
Zdroj: Molecular Microbiology
Molecular Microbiology, Wiley, 2016, 99 (4), pp.778-793. ⟨10.1111/mmi.13265⟩
Molecular Microbiology, 2016, 99 (4), pp.778-793. ⟨10.1111/mmi.13265⟩
ISSN: 0950-382X
1365-2958
DOI: 10.1111/mmi.13265⟩
Popis: International audience; The causative agent of Legionnaires' disease, Legionella pneumophila, employs the autoinducer compound LAI-1 (3-hydroxypentadecane-4-one) for cell-cell communication. LAI-1 is produced and detected by the Lqs (Legionella quorum sensing) system, comprising the autoinducer synthase LqsA, the sensor kinases LqsS and LqsT, as well as the response regulator LqsR. Lqs-regulated processes include pathogen-host interactions, production of extracellular filaments and natural competence for DNA uptake. Here we show that synthetic LAI-1 promotes the motility of L. pneumophila by signalling through LqsS/LqsT and LqsR. Upon addition of LAI-1, autophosphorylation of LqsS/LqsT by [γ-(32) P]-ATP was inhibited in a dose-dependent manner. In contrast, the Vibrio cholerae autoinducer CAI-1 (3-hydroxytridecane-4-one) promoted the phosphorylation of LqsS (but not LqsT). LAI-1 did neither affect the stability of phospho-LqsS or phospho-LqsT, nor the dephosphorylation by LqsR. Transcriptome analysis of L. pneumophila treated with LAI-1 revealed that the compound positively regulates a number of genes, including the non-coding RNAs rsmY and rsmZ, and negatively regulates the RNA-binding global regulator crsA. Accordingly, LAI-1 controls the switch from the replicative to the transmissive growth phase of L. pneumophila. In summary, the findings indicate that LAI-1 regulates motility and the biphasic life style of L. pneumophila through LqsS- and LqsT-dependent phosphorylation signalling.
Databáze: OpenAIRE
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