Growth and dissemination of human malignant lymphoblasts in immunosuppressed nu/nu mice
| Autor: | Cavallo, F., Riccardi, C., Forni, M., Pericle, F., Bosco, M. C., Mirella Giovarelli, Soleti, A., Forni, G. |
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| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
Cytotoxicity
Immunologic Immunosuppression Therapy Brain Neoplasms Transplantation Heterologous Mice Nude G(M1) Ganglioside Kidney Neoplasms Killer Cells Natural Mice Liver Neoplasms Experimental Poly I-C Bone Marrow Injections Intravenous Tumor Cells Cultured Animals Humans Leukemia-Lymphoma Adult T-Cell Female Neoplasm Transplantation |
| Zdroj: | Scopus-Elsevier |
| Popis: | Athymic nu/nu mice are commonly employed for the heterotransplantation of solid human tumors. Leukemias, however, have consistently proved difficult to transplant and, to enhance their take, recipient nu/nu mice have been variously immunosuppressed. In this study, the natural reactivity against human malignant T lymphoblast (PF382) of splenectomized nu/nu mice (S-nu/nu), nu/nu mice splenectomized and treated with polyinosinic-polycytidylic acid (SIC-nu/nu), and nu/nu mice splenectomized, irradiated and repeatedly injected with antiasialo GM1 antiserum (SIA-nu/nu) has been correlated with the in vivo growth of subcutaneous and intravenous PF382 cell challenges. SIC-nu/nu mice display a marked natural killer (NK) activity, quickly clear 125I-Urd-labelled PF382 cells injected intravenously and do not allow the growth of subcutaneous nor intravenous PF382 cell challenges. S-nu/nu mice display a slightly lower NK activity and slower clearance of 125I-Urd-labelled PF382 cells. Moreover, an intravenous PF382 cell challenge kills 56% of S-nu/nu mice. SIA-nu/nu mice have no NK activity, slowly clear 125I-Urd-labelled PF382 cells and always allow the growth of PF382 cells injected either subcutaneously or intravenously with a consistent pattern. Following the intravenous challenge, PF382 cells first metastasize to liver and kideny, then focal or diffuse infiltrations of the bone marrow and menings become evident. SIA-nu/nu mice thus offer an interesting experimental model for study of the pathogenesis of leukemic infiltration of the meninges, and the exploration of possible therapeutic approaches. |
| Databáze: | OpenAIRE |
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