Pathophysiological functions of CD30+ CD4+ T cells in rheumatoid arthritis
| Autor: | Okamoto, Akira, Yamamura, Masahiro, Iwahashi, Mitsuhiro, Aita, Tetsushi, Ueno, Akiko, Kawashima, Masanori, Yamana, Jiro, Kagawa, Hidetoshi, Makino, Hirofumi |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Gene Expression Ki-1 Antigen Apoptosis CD4 Tcells Arthritis Rheumatoid immune system diseases hemic and lymphatic diseases Humans rheumatoid arthritis(RA) Aged Aged 80 and over integumentary system Synovial Membrane NF-kappa B interleukin-4(IL=4) Middle Aged Solubility CD30 CD4 Antigens Female Interleukin-4 Cell Division Signal Transduction |
| Zdroj: | Acta medica Okayama. 57(6) |
| ISSN: | 0386-300X |
| Popis: | High levels of soluble CD30 (sCD30) were detected in the serum and synovial fluid of patients with rheumatoid arthritis (RA), indicating the involvement of CD30+ T cells in the pathogenesis. We investigated the induction of CD30 and its functions in CD4+T cells from patients with established RA (disease duration >_2 years). CD4+ T cells from both the peripheral blood (PB) and synovial tissue (ST) of RA patients expressed surface CD30 when stimulated with anti-CD3 antibody (Ab) and anti-CD28 Ab, but their CD30 induction was slower and weaker compared with PB CD4+ T cells of healthy controls (HC). Immunohistochemical analysis showed that only a small proportion of lymphocytes expressed CD30 in the ST (-1%). RA PB CD4+ T cells, after recovery from 6-day stimulation with anti-CD3 Ab and anti-CD28 Ab, showed in intracellular cytokine staining that CD30+ T cells could produce more interleukin-4 (IL-4) but less interferon-gamma. In the culture of RA PB CD4+ T Cells with anti-CD3 Ab and anti-CD28 Ab, blocking anti-CD30 Ab similarly inhibited the cell proliferation and activation of nuclear factor-kappaB on day 4 in RA and HC, but inhibited the apoptotic cell death on day 6 only in RA. These results indicate that despite high-level expression of sCD30, the anti-inflammatory activity of IL-4-producing CD30+ CD4+ T cells may be limited in the ST due to a poor induction of surface CD30 and a susceptibility to CD30-mediated cell death. |
| Databáze: | OpenAIRE |
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