Micropillar substrates: a tool for studying cell mechanobiology
| Autor: | Gupta, Mukund, Kocgozlu, Leyla, Sarangi, Bibhu Ranjan, Margadant, Felix, Ashraf, Mohammed, Ladoux, Benoit |
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| Přispěvatelé: | Mechanobiology Institute [Singapore] (MBI), National University of Singapore (NUS), Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Agence Nationale de la Recherche (ANR 2010 BLAN 1515 and Program Nanotechnologies and Nanosystems ANR-13-NANO-0011), Human Frontier Science Program (grant RGP0040/2012), Mechanobiology Institute, European Project: 617233,EC:FP7:ERC,ERC-2013-CoG,DURACELL(2014) |
| Rok vydání: | 2015 |
| Předmět: |
Mechanotransduction
MESH: Mechanotransduction Cellular Cell Survival Micropillar substrates Biophysics Force measurement [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biosensing Techniques Fibroblasts MESH: Image Processing Computer-Assisted Mechanotransduction Cellular MESH: Cell Adhesion [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biophysics MESH: Cell Survival MESH: Fibroblasts Rigidity Cell Adhesion Image Processing Computer-Assisted Animals MESH: Animals Traction force microscopy MESH: Biophysics MESH: Biosensing Techniques |
| Zdroj: | Methods in Cell Biology Methods in Cell Biology, Elsevier, 2014, 125, pp.289-308. ⟨10.1016/bs.mcb.2014.10.009⟩ Methods in Cell Biology, 2014, 125, pp.289-308. ⟨10.1016/bs.mcb.2014.10.009⟩ |
| ISSN: | 0091-679X |
| Popis: | International audience; Increasing evidence has shown that mechanical cues from the environment play an important role in cell biology. Mechanotransduction or the study of how cells can sense these mechanical cues, and respond to them, is an active field of research. However, it is still not clear how cells sense and respond to mechanical cues. Thus, new tools are being rapidly developed to quantitatively study cell mechanobiology. Particularly, force measurement tools such as micropillar substrates have provided new insights into the underlying mechanisms of mechanosensing. In this chapter, we provide detailed protocol for fabrication, characterization, functionalization, and use of the micropillar substrates. |
| Databáze: | OpenAIRE |
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