Shifts in cell-associated HIV-1 RNA but not in episomal HIV-1 DNA correlate with new cycles of HIV-1 infection in vivo
| Autor: | Marek, Fischer, Alexandra, Trkola, Beda, Joos, Roland, Hafner, Helen, Joller, Mark A, Muesing, David R, Kaufman, Esther, Berli, Bernard, Hirschel, Rainer, Weber, Huldrych F, Günthard |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
CD4-Positive T-Lymphocytes
Cell Extracts Anti-HIV Agents HIV Infections Virus Replication HIV-1/chemistry/ genetics Humans Longitudinal Studies Prospective Studies Biological Markers/blood Cells Cultured HIV Long Terminal Repeat ddc:616 Anti-HIV Agents/administration & dosage/ therapeutic use virus diseases DNA Viral/ blood Leukocytes Mononuclear/ virology CD4-Positive T-Lymphocytes/drug effects/virology DNA Viral HIV-1 Leukocytes Mononuclear RNA Viral Cell Extracts/genetics Drug Therapy Combination RNA Viral/ blood Biomarkers HIV Infections/blood/ drug therapy |
| Zdroj: | Antiviral Therapy, Vol. 8, No 2 (2003) pp. 97-104 |
| ISSN: | 1359-6535 |
| Popis: | The significance of distinct classes of HIV-1 nucleic acids as correlates of recent HIV-1 replication was assessed in peripheral blood mononuclear cells (PBMC) obtained from 14 patients during 2 weeks of structured interruption of antiretroviral therapy (STI) and 2 weeks of resuming therapy. Levels of HIV RNA in plasma (HIV-RNAplasma) and of unspliced cell-associated HIV-1 RNA (HIV-UsRNAPBMC) were significantly increased as a result of STI, whereas no significant shifts in the levels of 2-LTR episomal HIV-1 DNA (2-LTR circles) and total late HIV-1 reverse transcripts (late-DNA) were observed. Thus, limited viral replication had occurred, which had no effect on the pool size of infected cells in the periphery. Levels of 2-LTR circles did not reflect rapid changes in HIV-1 replication. In contrast, expression of HIV-UsRNAPBMC increased during STI and consequently provides a more sensitive, albeit not absolute cellular marker of ongoing HIV-1 replication. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|