Relationship between sperm chromatin status and ICSI outcome in men with obstructive azoospermia and unexplained infertile normozoospermia

Autor:	Mohamad Reza Sadeghi, Lakpour, N., Heidari-Vala, H., Hodjat, M., Amirjannati, N., Jadda, H. H., Binaafar, S., Akhondi, M. M.
Rok vydání:	2011
Předmět:	Adult Male Treatment Outcome Fertilization Humans Ejaculation DNA Fragmentation Sperm Injections Intracytoplasmic Spermatozoa Chromatin Azoospermia
Zdroj:	Europe PubMed Central Scopus-Elsevier
ISSN:	2066-8279
Popis:	This study was done to evaluate the effect of sperm source on chromatin integrity and ICSI outcomes. One hundred and thirteen samples containing epididymal aspirates of 57 obstructive azoospermic men and 56-ejaculated semen of normozoospermic men were included in this study. Sperm chromatin status was evaluated by Chromomycin A3 (CMA3), Aniline Blue (AB) and Toluidine Blue (TB). Fertilization rate and embryo quality were recorded. In epididymal group the percentage of sperms stained with AB, CMA3 and TB were significantly higher compared to ejaculate group while fertilization rate (60.6% vs. 74.04%) was significantly lower. However, embryo quality was not significantly different between two groups. In addition, abnormal sperm chromatin condensation and DNA fragmentation were not correlated with fertilization rate and embryo quality. Our results highlight the role of epididymis in sperm maturation and confirm that ICSI using ejaculated sperm is the gold standard for treatment of infertile men.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::6fdeb506ab82b009393e65fa5d9befb4 https://pubmed.ncbi.nlm.nih.gov/21655656 Zobrazit plný text záznamu