Antidegenerative effects of Mg(2+)-valproate in cultured cerebellar neurons
| Autor: | Bruno, V., Sortino, M. A., Scapagnini, U., Ferdinando NICOLETTI, Canonico, P. L. |
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| Rok vydání: | 1995 |
| Předmět: |
chemistry/drug effects
Free Radicals analysis Cells Neurotoxins Glutamic Acid Receptors N-Methyl-D-Aspartate analysis/pharmacology Cerebellum Receptors Animals Magnesium Cyclic GMP Cells Cultured Neurons Cultured Valproic Acid metabolism/pharmacology/toxicity analysis/biosynthesis Rats drug effects Nerve Degeneration Animals Calcium metabolism Cells Cultured Cerebellum chemistry/drug effects Cyclic GMP analysis/biosynthesis Free Radicals analysis Glutamic Acid metabolism/pharmacology/toxicity Magnesium analysis/pharmacology Nerve Degeneration drug effects Neurons chemistry/drug effects Neurotoxins biosynthesis Rats Receptors N-Methyl-D-Aspartate drug effects Transfer RNA Aminoacylation drug effects Valproic Acid Calcium Transfer RNA Aminoacylation biosynthesis metabolism |
| Zdroj: | Europe PubMed Central Scopus-Elsevier |
| Popis: | We have investigated in the present study the effect of Mg(2+)-valproate on necrotic degeneration induced by an excitotoxic insult in primary culture of cerebellar neurons, that is an homogeneous population of glutamatergic neurons. Mg(2+)-valproate protected cultures against glutamate-induced neurotoxicity, acting as an indirect N-methyl-D-aspartate (NMDA) receptor antagonist, thus reducing free radical formation and affecting the biochemical parameters (i.e. 45Ca(2+)-influx, cyclic GMP formation, inositol phospholipid hydrolysis and protein kinase C translocation) that undergo modifications following NMDA receptor activation in cerebellar granule cells. |
| Databáze: | OpenAIRE |
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