Adjuvant effects of CpG oligodeoxynucleotides on responses against T-independent type 2 antigens

Autor: Kovarik, Jiri, Bozzotti, P, Tougne, Chantal, Davis, HL, Lambert, Paul Henri, Krieg, AM, Siegrist, Claire-Anne
Jazyk: angličtina
Rok vydání: 2001
Předmět:
CD4-Positive T-Lymphocytes
Aging
Oligonucleotides
CpG Islands/immunology
ddc:616.07
Lymphocyte Activation/immunology
Lymphocyte Activation
Pneumococcal Vaccines
Mice
Adjuvants
Immunologic
Pneumococcal Vaccines/immunology
Animals
Ficoll
Carrier Proteins/immunology
Oligonucleotides/immunology
Streptococcus pneumoniae/immunology
Aging/immunology
Trinitrobenzenes/immunology
B-Lymphocytes
Mice
Inbred BALB C
ddc:618
Immunoglobulin M/biosynthesis
Ficoll/analogs & derivatives/immunology
Antigens
T-Independent/immunology
B-Lymphocytes/immunology
hemic and immune systems
Original Articles
respiratory system
Antibodies
Bacterial
Antigens
T-Independent
Streptococcus pneumoniae
Immunoglobulin M
Immunoglobulin G
Trinitrobenzenes
CD4-Positive T-Lymphocytes/immunology
Antibodies
Bacterial/biosynthesis
Haptens/immunology
CpG Islands
Carrier Proteins
Haptens
Immunoglobulin G/biosynthesis
Zdroj: Immunology, Vol. 102, No 1 (2001) pp. 67-76
ISSN: 0019-2805
Popis: Oligodeoxynucleotides containing CpG motifs (CpG-ODN) are potent in vitro B-cell activators and they have been successfully used to increase in vivo antibody responses to T-dependent peptide and protein antigens. In contrast, the use of CpG-ODN to enhance in vivo antibody responses to various T-independent type 2 (TI-2) antigens has recently generated contradictory results. In this study, we compared the CpG-ODN stimulatory effect on antibody responses of adult and young BALB/c mice to trinitrophenylaminoethyl-carboxymethyl (TNP) -Ficoll and to polysaccharides (PS) from several distinct serotypes of Streptococcus pneumoniae (SPn). CpG-ODN co-administration significantly enhanced antigen-specific immunoglobulin M (IgM), IgG, IgG1 and IgG2a titres to TNP-Ficoll. The depletion of CD4+ cells by monoclonal antibodies (GK1.5) identified their essential role in CpG-ODN-mediated enhancement of antibody responses. In contrast to TNP-Ficoll, CpG-ODN failed to enhance IgM and IgG responses to any of the 18 SPnPS serotypes tested. Providing T-cell epitopes by the conjugation of SPnPS to the carrier protein tetanus toxoid again allowed CpG-ODN to mediate enhancement of IgG, IgG2a and IgG3 responses to most SPnPS serotypes. Thus, antigen-presenting cell/T-cell interaction appears to largely mediate the in vivo influence of CpG-ODN on antibody responses to TI-2 antigens. In early life, additional factors limit CpG-ODN modulation of antibody responses to TI-2 antigens.
Databáze: OpenAIRE
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