DNA Damage Induced in Glioblastoma Cells by I-131: A Comparison between Experimental Data and Monte Carlo Simulation

Autor:	Neshasteh-Riz, A., Koosha, F., Mohsenifar, A., Seied Rabi Mahdavi
Jazyk:	angličtina
Rok vydání:	2012
Předmět:	Medical Physics Iodine-131 Radiology Glioblastoma Monte Carlo Method Research Article DNA Damage
Zdroj:	Cell Journal (Yakhteh) Scopus-Elsevier
ISSN:	2228-5814 2228-5806
Popis:	Objective: The passage of ionizing radiation in living cells creates clusters of damaged nucleotides in DNA. In this study, DNA strand breaks induced by the beta particle of iodine-131 (I-131), have been determined experimentally and compared to Monte Carlo simulation results as a theoretical method of determining131I damage. Materials and Methods: In this experimental study, in order to create single strand breaks (SSB) and double strand breaks (DSB) in the DNA, glioblastoma (GBM) cells were exposed to 10 mCi I-131, at a dose of 2 Gy. Damage of irradiated cells were evaluated quantitatively by the Fast Micromethod assay. The energy spectrum of electrons released in cells were obtained by the macroscopic Monte Carlo code (MCNP4c) and used as an input of the micro Monte Carlo code (MCDS). The percent of damage induced in cells was analyzed by Mann-Whitney test. Results: A significant reduction (p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::6e4fc3b6b91e79823fd0fe7b431fd1ad http://europepmc.org/articles/PMC3635817 Zobrazit plný text záznamu Plný text ve formátu PDF