Negative autoregulation of the Arabidopsis homeobox gene ATHB-2
| Autor: | Ohgishi M. (1), Oka A. (1), Morelli G. (2), Ruberti I. (3), Aoyama T. (1) |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Homeodomain Proteins
Base Sequence DNA Plant Arabidopsis Proteins Reverse Transcriptase Polymerase Chain Reaction autoregulation Arabidopsis DNA Footprinting Genes Homeobox Blotting Northern Plants Genetically Modified Recombinant Proteins DNA-Binding Proteins HD-Zip Gene Expression Regulation Plant ATHB-2 Transgenes transcription Promoter Regions Genetic Plant Proteins Protein Binding |
| Zdroj: | Plant journal 25 (2001): 389–397. doi:10.1046/j.1365-313x.2001.00966.x info:cnr-pdr/source/autori:Ohgishi M. (1), Oka A. (1), Morelli G. (2), Ruberti I. (3), Aoyama T. (1)/titolo:Negative autoregulation of the Arabidopsis homeobox gene ATHB-2/doi:10.1046%2Fj.1365-313x.2001.00966.x/rivista:Plant journal (Print)/anno:2001/pagina_da:389/pagina_a:397/intervallo_pagine:389–397/volume:25 |
| ISSN: | 0960-7412 |
| DOI: | 10.1046/j.1365-313x.2001.00966.x |
| Popis: | The Arabidopsis homeobox gene ATHB-2 is tightly regulated by light signals, and is thought to direct morphological changes during shade avoidance responses. To understand how ATHB-2 mediates light signals in plant morphogenesis, we investigated its transcriptional network. We constructed a gene encoding a chimeric transcription factor (HD-Zip-2-V-G) that is expected to activate target genes of ATHB-2 in a glucocorticoid-dependent manner. In transgenic Arabidopsis plants expressing HD-Zip-2-V-G, glucocorticoid treatment activates the ATHB-2 gene itself, independent of de novo protein synthesis. An in vitro DNase I-footprinting experiment showed that recombinant ATHB-2 protein specifically bound to an ATHB-2 promoter region. These complementary results indicate that ATHB-2 recognizes its own promoter. Consistent with the fact that ATHB-2 itself has been shown to act as a repressor, expression of the endogenous ATHB-2 gene was repressed in transgenic plants overexpressing an ATHB-2 transgene. Moreover, target-gene analysis using the HD-Zip-2-V-G suggested that ATHB-2 recognizes other HD-Zip II subfamily genes. We conclude that ATHB-2 has a negative autoregulatory loop and may be involved in a complicated transcriptional network involving paralogous genes, as in the case with animal homeobox genes. |
| Databáze: | OpenAIRE |
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