Immunohistochemical and molecular analysis of bax, bcl-2 and p53 genes in laryngeal squamous cell carcinomas

Autor:	Nicola Fracchiolla, Capaccio, P., Carboni, N., Pagliari, A. V., Neri, A., Ronchett, D., Pruner, G., Silvotti, M. G., Pignataro, L., Buffa, R., Broich, G.
Předmět:	Aged 80 and over Male Middle Aged Genes p53 Immunohistochemistry Genes bcl-2 Proto-Oncogene Proteins c-bcl-2 Proto-Oncogene Proteins Mutation Carcinoma Squamous Cell Humans Female Tumor Suppressor Protein p53 Laryngeal Neoplasms Aged bcl-2-Associated X Protein
Zdroj:	Europe PubMed Central Scopus-Elsevier ResearcherID
Popis:	We investigated the role of apoptosis and its potential alterations in laryngeal squamous cell carcinomas (LSCCs) by evaluating bax, bcl-2 and p53 protein expression in 50 cases and by characterising the molecular status of the bax and p53 genes.p53 and bax gene mutations were investigated by means of PCR/SSCP and direct DNA sequencing, and bax, bcl-2 and p53 protein expression by means of immunohistochemistry.We identified p53 gene mutations in 17/50 cases (34%); p53 expression in 26 of the 50 cases (52%); bcl-2 expression in 5/50 cases (10%); bax expression in 32/47 cases (68%). 18/33 cases with a wild type p53 gene overexpressed p53 protein: 12 cases (approximately 66%) were bax+/bcl-2-. Of the remaining cases without p53 protein expression, seven cases (approximately 47%) were bax+/bcl-2-.Our observations suggest that the overexpression of p53 may contribute to the repression of bcl-2 and the induction of bax expression in LSCCs. However, the fact that a number of cases not expressing p53 did not present any clear up-regulation of bax or down-regulation of bcl-2 suggests that bcl-2 and bax may be regulated by various mechanisms other than p53.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::6de6df4be43996668d5faed12ff3c7dd http://europepmc.org/abstract/med/10368653 Zobrazit plný text záznamu