Nasal or throat sampling is adequate for the detection of the human respiratory syncytial virus in children with acute respiratory infections

Autor: Nguyen, V, Russell, F, Dance, D, Vilivong, K, Phommachan, S, Syladeth, C, Lai, J, Lim, R, Morpeth, M, Mayxay, M, Newton, P, De Lamballerie, X, Dubot-Pérès, A
Přispěvatelé: Unité des Virus Emergents (UVE), Aix Marseille Université (AMU)-Institut de Recherche pour le Développement (IRD)-Institut National de la Santé et de la Recherche Médicale (INSERM), Murdoch Children's Research Institute (MCRI), Department of Paediatrics [Melbourne], Melbourne Medical School [Melbourne], Faculty of Medicine, Dentistry and Health Sciences [Melbourne], University of Melbourne-University of Melbourne-Faculty of Medicine, Dentistry and Health Sciences [Melbourne], University of Melbourne-University of Melbourne, Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Mahidol University [Bangkok]-Mahosot Hospital, Nuffield Department of Clinical Medicine [Oxford], University of Oxford [Oxford], London School of Hygiene and Tropical Medicine (LSHTM), Institute of Research and Education Development [Vientiane, Lao People’s Democratic Republic], University of Health Sciences [Vientiane, Laos] (UHS), Murdoch Children’s Research Institute, European Union’s Horizon 2020 research and innovation programme EVAg, Grant/Award Number: 653316, Aix‐Marseille University, Bill & Melinda Gates Foundation, Grant/Award Number: OPP1115490, Wellcome Trust of Great Britain, Grant/Award Number: 089275/H/09/Z0, Institute of Research for Development, European Project: 653316,H2020,H2020-INFRAIA-2014-2015,EVAg(2015), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Oxford, BUISINE, Soline, European Virus Archive goes global - EVAg - - H20202015-04-01 - 2019-03-31 - 653316 - VALID
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Journal of Medical Virology
Journal of Medical Virology, Wiley-Blackwell, 2019, 91 (9), pp.1602-1607. ⟨10.1002/jmv.25496⟩
Journal of Medical Virology, 2019, 91 (9), pp.1602-1607. ⟨10.1002/jmv.25496⟩
ISSN: 0146-6615
1096-9071
DOI: 10.1002/jmv.25496⟩
Popis: International audience; Human respiratory syncytial virus (HRSV) is one of the most important causes of acute respiratory infections (ARI) in young children. HRSV diagnosis is based on the detection of the virus in respiratory specimens. Nasopharyngeal swabbing is considered the preferred method of sampling, although there is limited evidence of the superiority of nasopharyngeal swabs (NPS) over the less invasive nasal (NS) and throat (TS) swabs for virus detection by real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). In the current study, we compared the three swabbing methods for the detection of HRSV by RT-qPCR in children hospitalized with ARI at Mahosot Hospital, Vientiane, Laos. In 2014, NS, NPS, and TS were collected from 288 children. All three samples were tested for HRSV by RT-qPCR; 141 patients were found positive for at least one sample. Almost perfect agreements (κ > 0.8) between the swabs, compared two by two, were observed. Detection rates for the three swabs (between 93% and 95%) were not significantly different, regardless of the clinical presentation. Our findings suggest that the uncomfortable and technically more demanding NPS method is not mandatory for HRSV detection by RT-qPCR.
Databáze: OpenAIRE
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