H-NS is the major repressor of Salmonella Typhimurium Pef fimbriae expression
| Autor: | Hurtado-Escobar, Genaro Alejandro, Grépinet, Olivier, Raymond, Pierre, Abed, Nadia, Velge, Philippe, Virlogeux-Payant, Isabelle |
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| Přispěvatelé: | Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Institut National de la Recherche Agronomique (INRA), ERA-NET InfectERA (Sal host trop), Region Centre-Val de Loire (2008-00036085), Délégation Régionale à la Recherche et à la Technologie du Centre (FEDER) (1634-32245), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
H-NS
Transcription Genetic culture in vitro fimbriae lcsh:Infectious and parasitic diseases Bacterial Proteins Operon in vitro culture lcsh:RC109-216 pcr inverse Promoter Regions Genetic nucleoprotein bactérie régulation de l'expression génique western blot opéron nucléoprotéine nucleoproteins Gene Expression Regulation Bacterial Pef bacterium mécanisme de régulation [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology DNA-Binding Proteins Repressor Proteins [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Fimbriae Bacterial Salmonella Typhimurium bacteria Fimbriae Proteins Hha Research Paper Molecular Chaperones |
| Zdroj: | Virulence Virulence, Taylor & Francis, 2019, 10 (1), pp.849-867. ⟨10.1080/21505594.2019.1682752⟩ Virulence, Vol 10, Iss 1, Pp 849-867 (2019) |
| ISSN: | 2150-5594 2150-5608 |
| DOI: | 10.1080/21505594.2019.1682752⟩ |
| Popis: | International audience; Fimbriae play an important role in adhesion and are therefore essential for the interaction of bacteria with the environments they encounter. Most of them are expressed in vivo but not in vitro, thus making difficult the full characterization of these fimbriae. Here, we characterized the silencing of plasmid-encoded fimbriae (Pef) expression, encoded by the pef operon, in the worldwide pathogen Salmonella Typhimurium. We demonstrated that the nucleoid-associated proteins H-NS and Hha, and their respective paralogs StpA and YdgT, negatively regulate at pH 5.1 and pH 7.1 the transcription of the pef operon. Two promoters, PpefB and PpefA, direct the transcription of this operon. All the nucleoid-associated proteins silence the PpefB promoter and H-NS also targets the PpefA promoter. While Hha and YdgT are mainly considered as acting primarily through H-NS to modulate gene transcription, our results strongly suggest that Hha and YdgT silence pef transcription at acidic pH either by interacting with StpA or independently of H-NS and StpA. We also confirmed the previously described post-transcriptional repression of Pef fimbriae by CsrA titration via the fim mRNA and CsrB and CsrC sRNA. Finally, among all these regulators, H-NS clearly appeared as the major repressor of Pef expression. These results open new avenues of research to better characterize the regulation of these bacterial adhesive proteins and to clarify their role in the virulence of pathogens |
| Databáze: | OpenAIRE |
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