Global impact of mature biofilm lifestyle on Escherichia coli K-12 gene expression

Autor: Beloin, C., Valle, J., Latour-Lambert, P., Faure, P., Kzreminski, M., Balestrino, D., Janus Anders Juul Haagensen, Søren Molin, Prensier, G., Arbeille, B., Ghigi, J. M.
Přispěvatelé: Génétique des Biofilms, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Universidad Pública de Navarra [Espagne] = Public University of Navarra (UPNA), Récepteurs et Cognition (RC), Collège de France (CdF (institution))-Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Université d'Auvergne - Clermont-Ferrand I (UdA), BioCentrum-DTU (BiC), Danmarks Tekniske Universitet = Technical University of Denmark (DTU), Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Faculté de Médecine, Laboratoire Biologie Cellulaire et Microscopie Electronique, Université Francois Rabelais [Tours], This work was supported by grants from the ‘PRFMMIP – Réseau Infections Nosocomiales’ and the Institut Pasteur., We thank Claude Lebos for preparation of the SEM micrographs. We are grateful to E. Krin for assistance in macroarray procedure. We thank T. Pugsley and C. Dorel for the kind gift of some strains used in this study, and A. Idja, J. Bellalou and R. Longin (PT5, Institut Pasteur) for technical assistance. We thank S. Da Re, B. Lakowski, C. Buchrieser, U. Dobrindt, T. Msadek, I. Lasa, M. Swanson and P. Delepelaire for critical reading of the manuscript. We also thank the referees for their helpful suggestions., Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Collège de France (CdF (institution))-Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Technical University of Denmark [Lyngby] (DTU)
Jazyk: angličtina
Rok vydání: 2004
Předmět:
Zdroj: Molecular Microbiology
Molecular Microbiology, 2004, 51 (3), pp.659-74. ⟨10.1046/j.1365-2958.2003.03865.x⟩
Molecular Microbiology, Wiley, 2004, 51 (3), pp.659-74. ⟨10.1046/j.1365-2958.2003.03865.x⟩
Technical University of Denmark Orbit
ISSN: 0950-382X
1365-2958
DOI: 10.1046/j.1365-2958.2003.03865.x⟩
Popis: International audience; The formation of biofilm results in a major lifestyle switch that is thought to affect the expression of multiple genes and operons. We used DNA arrays to study the global effect of biofilm formation on gene expression in mature Escherichia coli K-12 biofilm. We show that, when biofilm is compared with the exponential growth phase, 1.9% of the genes showed a consistent up- or downregulation by a factor greater than two, and that 10% of the E. coli genome is significantly differentially expressed. The functions of the genes induced in these conditions correspond to stress response as well as energy production, envelope biogenesis and unknown functions. We provide evidence that the expression of stress envelope response genes, such as the psp operon or elements of the cpx and rpoE pathways, is a general feature of E. coli mature biofilms. We also compared biofilm with the stationary growth phase and showed that the biofilm lifestyle, although sharing similarities with the stationary growth phase, triggers the expression of specific sets of genes. Using gene disruption of 54 of the most biofilm-induced genes followed by a detailed phenotypic study, we validated the biological relevance of our analysis and showed that 20 of these genes are required for the formation of mature biofilm. This group includes 11 genes of previously unknown function. These results constitute a comprehensive analysis of the global transcriptional response triggered in mature E. coli biofilms and provide insights into its physiological signature.
Databáze: OpenAIRE
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