Nitric oxide (NO) and cartilage metabolism: NO effects are modulated by superoxide in response to IL-1

Autor: Jouzeau, Jean-Yves, Pacquelet, Sandrine, Boileau, Christelle, Nédélec, Emmanuelle, Presle, Nathalie, Netter, Patrick, Terlain, Bernard
Přispěvatelé: Centre de recherche du Chum [Montréal] (CRCHUM), Centre Hospitalier de l'Université de Montréal (CHUM), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Laboratoire de physiologie et Pathologie articulaire, Faculte de Medecine, Vandoeuvre-les-Nancy, Université Henri Poincaré - Nancy 1 (UHP), Physiopathologie, Pharmacologie et Ingénierie articulaires (PPIA), Université Henri Poincaré - Nancy 1 (UHP)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CR CHUM), Université de Montréal (UdeM)-Université de Montréal (UdeM), Unité de Recherche en Arthrose, Centre de Recherche de l'Université de Montréal ( CR-CHUM ), Université de Montréal, Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Université Henri Poincaré - Nancy 1 ( UHP ), Physiopathologie, Pharmacologie et Ingénierie articulaires ( PPIA ), Université Henri Poincaré - Nancy 1 ( UHP ) -Centre National de la Recherche Scientifique ( CNRS )
Rok vydání: 2002
Předmět:
Cartilage
Articular

Male
[ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition
MESH: Interleukin-17
Wistar
MESH : Depression
Chemical

MESH: omega-N-Methylarginine
Models
MESH: Animals
Enzyme Inhibitors
Cells
Cultured

Cultured
MESH: Salicylic Acids
MESH : Rats
Depression
Interleukin-17
MESH : Cartilage
Articular

Free Radical Scavengers
Salicylates
MESH : Proteoglycans
MESH : Nitric Oxide
MESH: Models
Animal

MESH: Enzyme Inhibitors
MESH: Proteoglycans
Depression
Chemical

Models
Animal

MESH: Nitric Oxide Synthase
Proteoglycans
Cartilage Diseases
MESH : Free Radical Scavengers
MESH: Cells
Cultured

MESH: Rats
MESH : Male
Cells
Chemical
MESH : Interleukin-17
MESH : Rats
Wistar

Nitric Oxide
MESH : Interleukin-1
MESH : Chondrocytes
Chondrocytes
MESH: Chondrocytes
MESH : Cells
Cultured

Animals
Humans
Rats
Wistar

MESH : Enzyme Inhibitors
MESH: Depression
Chemical

MESH: Humans
omega-N-Methylarginine
Animal
MESH : Humans
MESH : Nitric Oxide Synthase
MESH: Cartilage Diseases
MESH: Interleukin-1
MESH: Rats
Wistar

MESH : Models
Animal

MESH: Male
Rats
MESH : Salicylic Acids
MESH : Cartilage Diseases
Cartilage
MESH: Nitric Oxide
MESH: Cartilage
Articular

MESH: Free Radical Scavengers
MESH : omega-N-Methylarginine
MESH : Animals
Salicylic Acids
Nitric Oxide Synthase
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Articular
Interleukin-1
Zdroj: Biorheology
Biorheology, IOS Press, 2002, 39 (1-2), pp.201-14
ISSN: 0006-355X
Popis: International audience; Nitric oxide (NO) is thought to mediate most effects of interleukin-1 (IL-1) on cartilage. In vitro evidence includes the decreased synthesis of extracellular matrix components, the abnormal cell renewal, the decreased production of IL-1 receptor antagonist, the induction of apoptosis and the enhanced sensitivity of chondrocytes to oxidative stress. Studies in NOS2(-/-) mice or administration of NO synthase inhibitors in animal models of joint disorders have confirmed its potent pathophysiological role in cartilage. Using L-NMMA (1 mM), as a NO synthase inhibitor, and CuDips (10 microM), as a SOD mimetic, we provide evidence that the inhibitory potency of IL-1beta on proteoglycan synthesis and its stimulating effect on COX-2 activity depend both on NO and O2-* production. Peroxynitrite formation is further demonstrated by the occurrence of 3-nitrotyrosines in chondrocytes stimulated in vitro with 2.5 ng/ml IL-1 and in femoral condyles of rats injected locally with 1 microg IL-1. Preliminary data suggest that such contribution of reactive oxygen species is not shared in common by IL-17, another NO-producing cytokine. We conclude that superoxide is a key modulator of NO-mediated effects in chondrocyte stimulated with IL-1 and that a combined therapy with NO synthase inhibitors and antioxidants may be promising for a full cartilage protection.
Databáze: OpenAIRE
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