Nitric oxide (NO) and cartilage metabolism: NO effects are modulated by superoxide in response to IL-1
Autor: | Jouzeau, Jean-Yves, Pacquelet, Sandrine, Boileau, Christelle, Nédélec, Emmanuelle, Presle, Nathalie, Netter, Patrick, Terlain, Bernard |
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Přispěvatelé: | Centre de recherche du Chum [Montréal] (CRCHUM), Centre Hospitalier de l'Université de Montréal (CHUM), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Laboratoire de physiologie et Pathologie articulaire, Faculte de Medecine, Vandoeuvre-les-Nancy, Université Henri Poincaré - Nancy 1 (UHP), Physiopathologie, Pharmacologie et Ingénierie articulaires (PPIA), Université Henri Poincaré - Nancy 1 (UHP)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CR CHUM), Université de Montréal (UdeM)-Université de Montréal (UdeM), Unité de Recherche en Arthrose, Centre de Recherche de l'Université de Montréal ( CR-CHUM ), Université de Montréal, Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Université Henri Poincaré - Nancy 1 ( UHP ), Physiopathologie, Pharmacologie et Ingénierie articulaires ( PPIA ), Université Henri Poincaré - Nancy 1 ( UHP ) -Centre National de la Recherche Scientifique ( CNRS ) |
Rok vydání: | 2002 |
Předmět: |
Cartilage
Articular Male [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition MESH: Interleukin-17 Wistar MESH : Depression Chemical MESH: omega-N-Methylarginine Models MESH: Animals Enzyme Inhibitors Cells Cultured Cultured MESH: Salicylic Acids MESH : Rats Depression Interleukin-17 MESH : Cartilage Articular Free Radical Scavengers Salicylates MESH : Proteoglycans MESH : Nitric Oxide MESH: Models Animal MESH: Enzyme Inhibitors MESH: Proteoglycans Depression Chemical Models Animal MESH: Nitric Oxide Synthase Proteoglycans Cartilage Diseases MESH : Free Radical Scavengers MESH: Cells Cultured MESH: Rats MESH : Male Cells Chemical MESH : Interleukin-17 MESH : Rats Wistar Nitric Oxide MESH : Interleukin-1 MESH : Chondrocytes Chondrocytes MESH: Chondrocytes MESH : Cells Cultured Animals Humans Rats Wistar MESH : Enzyme Inhibitors MESH: Depression Chemical MESH: Humans omega-N-Methylarginine Animal MESH : Humans MESH : Nitric Oxide Synthase MESH: Cartilage Diseases MESH: Interleukin-1 MESH: Rats Wistar MESH : Models Animal MESH: Male Rats MESH : Salicylic Acids MESH : Cartilage Diseases Cartilage MESH: Nitric Oxide MESH: Cartilage Articular MESH: Free Radical Scavengers MESH : omega-N-Methylarginine MESH : Animals Salicylic Acids Nitric Oxide Synthase [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Articular Interleukin-1 |
Zdroj: | Biorheology Biorheology, IOS Press, 2002, 39 (1-2), pp.201-14 |
ISSN: | 0006-355X |
Popis: | International audience; Nitric oxide (NO) is thought to mediate most effects of interleukin-1 (IL-1) on cartilage. In vitro evidence includes the decreased synthesis of extracellular matrix components, the abnormal cell renewal, the decreased production of IL-1 receptor antagonist, the induction of apoptosis and the enhanced sensitivity of chondrocytes to oxidative stress. Studies in NOS2(-/-) mice or administration of NO synthase inhibitors in animal models of joint disorders have confirmed its potent pathophysiological role in cartilage. Using L-NMMA (1 mM), as a NO synthase inhibitor, and CuDips (10 microM), as a SOD mimetic, we provide evidence that the inhibitory potency of IL-1beta on proteoglycan synthesis and its stimulating effect on COX-2 activity depend both on NO and O2-* production. Peroxynitrite formation is further demonstrated by the occurrence of 3-nitrotyrosines in chondrocytes stimulated in vitro with 2.5 ng/ml IL-1 and in femoral condyles of rats injected locally with 1 microg IL-1. Preliminary data suggest that such contribution of reactive oxygen species is not shared in common by IL-17, another NO-producing cytokine. We conclude that superoxide is a key modulator of NO-mediated effects in chondrocyte stimulated with IL-1 and that a combined therapy with NO synthase inhibitors and antioxidants may be promising for a full cartilage protection. |
Databáze: | OpenAIRE |
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