Regional Differences in the Permeability Barrier of the Skin-Implications in Acantholytic Skin Diseases
| Autor: | Anikó Kapitány, Barbara Medgyesi, Adrienn Jenei, Orsolya Somogyi, Lilla Szabó, Krisztián Gáspár, Gábor Méhes, Zoltán Hendrik, Klaudia Dócs, Péter Szücs, Zsolt Dajnoki, Andrea Szegedi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male QH301-705.5 tight junction keratinocyte Middle Aged Permeability Article Hailey–Hailey disease Tight Junctions Chemistry Sebaceous Glands Acantholysis Humans Female acantolytic diseases cornified envelope Biology (General) Epidermis Darier’s disease desmosome barrier function QD1-999 Aged |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 19 International Journal of Molecular Sciences, Vol 22, Iss 10428, p 10428 (2021) |
| ISSN: | 1422-0067 |
| Popis: | The chemical milieu, microbiota composition, and immune activity show prominent differences in distinct healthy skin areas. The objective of the current study was to compare the major permeability barrier components (stratum corneum and tight junction (TJ)), investigate the distribution of (corneo)desmosomes and TJs, and measure barrier function in healthy sebaceous gland-rich (SGR), apocrine gland-rich (AGR), and gland-poor (GP) skin regions. Molecules involved in cornified envelope (CE) formation, desquamation, and (corneo)desmosome and TJ organization were investigated at the mRNA and protein levels using qRT-PCR and immunohistochemistry. The distribution of junction structures was visualized using confocal microscopy. Transepidermal water loss (TEWL) functional measurements were also performed. CE intracellular structural components were similarly expressed in gland-rich (SGR and AGR) and GP areas. In contrast, significantly lower extracellular protein levels of (corneo)desmosomes (DSG1 and CDSN) and TJs (OCLN and CLDN1) were detected in SGR/AGR areas compared to GP areas. In parallel, kallikrein proteases were significantly higher in gland-rich regions. Moreover, gland-rich areas were characterized by prominently disorganized junction structures ((corneo)desmosomes and TJs) and significantly higher TEWL levels compared to GP skin, which exhibited a regular distribution of junction structures. According to our findings, the permeability barrier of our skin is not uniform. Gland-rich areas are characterized by weaker permeability barrier features compared with GP regions. These findings have important clinical relevance and may explain the preferred localization of acantholytic skin diseases on gland-rich skin regions (e.g., Pemphigus foliaceus, Darier’s disease, and Hailey–Hailey disease). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|