| Autor: |
Shao-Ru, Wang, Wei, Sun, Nan, Zhou, Kai, Zhao, Wen-Jian, Li, Zeng-Peng, Chi, Ying, Wang, Qi-Min, Wang, Lei, Tong, Zong-Xuan, He, Hong-Yu, Han, Zheng-Gang, Chen |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Hua Xi Kou Qiang Yi Xue Za Zhi |
| ISSN: |
2618-0456 |
| Popis: |
OBJECTIVE: This study aimed to explore the effects of silencing isoprenylcysteine carboxyl methyltransferase (Icmt) through small interfering RNA (siRNA) interference on the proliferation and apoptosis of tongue squamous cell carcinoma (TSCC). METHODS: Three siRNA were designed and constructed for the Icmt gene sequence and were then transfected into TSCC cells CAL-27 and SCC-4 to silence Icmt expression. The tested cells were divided as follows: RNA interference groups Icmt-siRNA-1, Icmt-siRNA-2, and Icmt-siRNA-3, negative control group, and blank control group. The transfection efficiency of siRNA was detected by the fluorescent group Cy3-labeled siRNA, and the expression of Icmt mRNA was screened by quantitive real-time polymerase chain reaction (qRT-PCR) selected the experimental group for subsequent experiments. The expression of Icmt, RhoA, Cyclin D1, p21, extracellular regulated protein kinases (ERK), and phospho-extracellular regulated protein kinases (p-ERK) were analyzed by Western blot. The proliferation abilities of TSCC cells were determined by cell counting kit-8 assay. The change in apoptosis was detected by AnnexinV-APC/propidium staining (PI) assay. Cell-cycle analysis was conducted by flow cytometry. RESULTS: The expression of Icmt mRNA and protein in TSCC cells significantly decreased after Icmt-siRNA transfection (P0.05), but the expression of RhoA membrane protein decreased compared with the negative control group and blank control groups (P0.05). However, the phosphorylation level of ERK was significantly reduced (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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