Microplate screening assay for the detection of arsenite-oxidizing and arsenate-reducing bacteria
| Autor: | Simeonova, Diliana D, Lièvremont, Didier, Lagarde, Florence, Muller, Daniel A E, Groudeva, Veneta I, Lett, Marie-Claire |
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| Přispěvatelé: | Dynamique, évolution et expression de génomes de microorganismes (DEEGM), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2004 |
| Předmět: |
MESH: Oxidation-Reduction
Bacteriological Techniques Bacteria Arsenites MESH: Silver Nitrate MESH: Bacteriological Techniques Buffers MESH: Research Support Non-U.S. Gov't Arsenic MESH: Bacteria [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Biodegradation Environmental MESH: Arsenic MESH: Colorimetry Arsenates Silver Nitrate Colorimetry MESH: Arsenites MESH: Biodegradation MESH: Buffers Oxidation-Reduction MESH: Arsenates |
| Zdroj: | Microbiology Letters Microbiology Letters, Wiley, 2004, 237 (2), pp.249-53. ⟨10.1016/j.femsle.2004.06.040⟩ |
| ISSN: | 0378-1097 |
| DOI: | 10.1016/j.femsle.2004.06.040⟩ |
| Popis: | An efficient, inexpensive microplate colorimetric assay for screening of bacteria which can be used in bioremediation of arsenic was developed. The assay is based on the colorimetric analysis of the precipitates formed upon reaction of silver nitrate with arsenic. The method proved reliable and sensitive for the detection of As[III] oxidizers and As[V] reducers and can be used over a large pH range (5.8-8.4). Seventy-eight bacterial strains isolated from different environments were tested by this method. It also showed agreement with results obtained by high-performance liquid chromatography coupled with inductively coupled plasma atomic emission spectrometry. |
| Databáze: | OpenAIRE |
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