NF-κB Activity Initiates Human ESC-Derived Neural Progenitor Cell Differentiation by Inducing a Metabolic Maturation Program
| Autor: | FitzPatrick, Lorna M., Hawkins, Kate E., Delhove, Juliette M.K.M., Fernández Sánchez, Emilio, Soldati, Chiara, Bullen, Louise F., Nohturfft, Axel, Waddington, Simon N., Medina, Diego L., Bolaños Hernández, Juan Pedro, McKay, Tristan R. |
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| Přispěvatelé: | FitzPatrick Lorna, M., Hawkins Kate, E., Delhove Juliette, M. K. M., Fernandez, Emilio, Soldati, Chiara, Bullen Louise, F., Nohturfft, Axel, Waddington Simon, N., Medina, D, Bolaños Juan, P., McKay Tristan, R., St George's, University of London, European Commission, European Research Council, Ministerio de Economía y Competitividad (España), Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable (España), Medical Research Council (UK) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
autophagy
desarrollo embrionario Neurogenesis 2407 Biología Celular Embryonic Development Models Biological Article Neural Stem Cells Autophagy Humans ciclo celular Cells Cultured Embryonic Stem Cells health care economics and organizations NF-κB signalingneurogenesis Gene Expression Profiling Cell Cycle NF-kappa B Computational Biology Cell Differentiation Immunohistochemistry Gene Ontology Phenotype Metabolism neurogénesis NF-κB signaling Human embryonic development Energy Metabolism human embryonic development metabolism Biomarkers Signal Transduction |
| Zdroj: | GREDOS. Repositorio Institucional de la Universidad de Salamanca instname Digital.CSIC. Repositorio Institucional del CSIC Stem Cell Reports GREDOS: Repositorio Institucional de la Universidad de Salamanca Universidad de Salamanca (USAL) |
| ISSN: | 2213-6711 |
| Popis: | Summary Human neural development begins at embryonic day 19 and marks the beginning of organogenesis. Neural stem cells in the neural tube undergo profound functional, morphological, and metabolic changes during neural specification, coordinated by a combination of exogenous and endogenous cues. The temporal cell signaling activities that mediate this process, during development and in the postnatal brain, are incompletely understood. We have applied gene expression studies and transcription factor-activated reporter lentiviruses during in vitro neural specification of human pluripotent stem cells. We show that nuclear factor κB orchestrates a multi-faceted metabolic program necessary for the maturation of neural progenitor cells during neurogenesis. Highlights • NF-κB activation is required during normal human neurogenesis • NPCs that have the capacity to mature into neurons have high NF-κB signaling • NPC maturation involves changes in metabolism, autophagy, and cell-cycle regulation • NF-κB activation inhibits the expression of neural stem cell markers In this research article, FitzPatrick and colleagues have highlighted the requirement for NF-κB signaling in neural specification of human embryonic stem cells. They demonstrate that its activity orchestrates a metabolic shift toward oxidative phosphorylation in committing neural progenitor cells. Moreover, they demonstrate that progenitor cells with increased endogenous NF-κB activity have a higher propensity for maturation. |
| Databáze: | OpenAIRE |
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