In vivo knock-down of multidrug resistance transporters ABCC1 and ABCC2 by AAV-delivered shrnas and by artificial miRNAs
| Autor: | Florie Borel, van Logtenstein R, Koornneef A, Maczuga P, Ritsema T, Petry H, Sj, Deventer, Pl, Jansen, Konstantinova P |
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| Přispěvatelé: | Other departments, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Gastroenterology and Hepatology |
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| Zdroj: | Scopus-Elsevier Journal of RNAi and gene silencing, 7, 434-442. Library Publishing Media Journal of RNAi and Gene Silencing : An International Journal of RNA and Gene Targeting Research Europe PubMed Central |
| ISSN: | 1747-0854 |
| Popis: | ABC transporters export clinically-relevant drugs and their over-expression causes multidrug resistance. In order to knock-down ABC transporters, ABCC1 and ABCC2, 13 shRNAs were developed. Four shRNA candidates were tested in vivo using self-complementary adeno-associated virus serotype 8. A strong, specific knock-down of Abbc2 was observed in mice liver, but at the cost of toxicity caused by oversaturation of the RNAi machinery due to high shRNA expression. Subsequent generation of artificial miRNAs showed better efficacy profile. These results demonstrate the feasibility of knocking down Abbc2 via AAV-delivered shRNAs to the liver, and encourage the use of miRNA in further therapeutics development. |
| Databáze: | OpenAIRE |
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