[Structure-activity organization of the variola virus genome. III. Sequencing and analysis of the nucleotide sequence of the conserved region of HindIII-F, -N-, and -A-fragments of the India 1967 strain genome]

Autor:	Sn, Shchelkunov, Sm, Resenchuk, Av, Totmenin, Aa, Kolykhalov, Iv, Frolov, Sm, Dryga, Viktor Volchkov, Ve, Chizhikov, Vv, Gutorov, Vm, Blinov
Předmět:	Base Sequence Molecular Sequence Data Restriction Mapping Virion DNA-Directed RNA Polymerases Deoxyribonuclease HindIII Genome Viral Variola virus Inclusion Bodies Viral Open Reading Frames Viral Proteins RNA Viral Amino Acid Sequence Conserved Sequence Transcription Factors
Zdroj:	Europe PubMed Central
Popis:	Computer analysis of variola major virus (VAR) genomic fragment bounded by open reading frames (ORFs) D1R and A33L which is 47,961 bp long revealed 46 potential ORFs. The VAR proteins were compared with the analogous proteins of vaccinia virus strain Copenhagen. The subunits of DNA-dependent RNA polymerase, as well as the transcription factors, mRNA capping enzymes, and proteins necessary for the virion morphogenesis proved to be highly conservative within orthopoxviruses. The most pronounced differences between the VAR genome fragment under study and the corresponding vaccinia virus fragment were revealed in the vicinity of the gene encoding the A-type inclusion body protein. The possible functions of the analyzed viral proteins are discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::6624f2b257187e144b2a5d6c4ab97d6f http://europepmc.org/abstract/med/8183271 Zobrazit plný text záznamu